摘要
目的探讨C57BL/6J小鼠随年龄增长耳蜗内毛细胞带状突触(ribbon synapses,RS)的数量变化情况。方法分别取2、6、10、12月龄C57BL/6J小鼠耳蜗基底膜(每组5只),应用免疫组织化学染色方法对RS前、后膜进行双重标记,激光共聚焦显微镜下定位成像,并应用三维重建技术对基底膜各段RS的数量进行计数。结果与2月龄小鼠相比较,6月龄C57BL/6J小鼠耳蜗基底膜RS数量减少主要发生在底回,10月龄时各回RS数量均明显减少,12月龄时顶回、中回RS数量继续减少,底回反而有少许增多。结论RS数量减少可能发生在C57BL/6J小鼠老年性聋的早期阶段,探索阻止RS数量减少的治疗措施可能成为早期干预老年性聋的重要途径。
Objective To investigate the number changes of the ribbon synapses（RS） in the inner hair cell of C57BL/6J mice with age .Methods The cochlear basilar membrane was obtained from C57BL/6J mice in 2 ,6 ,10 , 12 months old .RIBEYE on the presynaptic membrane and AMPA receptor on the postsynaptic membrane were double labeled by immunofluorescence histochemical technique .The stained sections were observed under a confocal laser-scanning microscope .Then the number of RS of the basilar membrane was calculated by three dimensional reconstruction images using 3DS max software .Results The numbers of RS reduced gradually from the cochlear a‐pex to base in the same developing stages .The numbers of RS in apex and middle turn of cochlea were gradually re‐duced with the age increasing .The number of RS in basilar turn reduced first and increased slowly then .Conclusion The decreases of the number of RS maybe the main pathological change at the early stage of presbycusis .In addi‐tion ,there may be a kind of compensatory mechanism by increaseing the number of RS to delay the hearing deficits .
出处
《听力学及言语疾病杂志》
CAS
CSCD
北大核心
2015年第2期176-180,共5页
Journal of Audiology and Speech Pathology
基金
北京市自然科学基金面上项目(7142155)
2013年度全军保健专项科研课题(13BJZ24)
全军十二五面上项目(11MS013)
总参军事医学和老年病科研基金重点项目(ZCW14B07)
中国人民解放军第309医院院内课题面上课题(2014MS-015)联合资助
