摘要
本文以Ca2+通道抑制剂LaCl3处理"秦冠"苹果成熟花粉,观察其对花粉管生长以及细胞壁成分的影响。结果发现较高浓度LaCl3处理花粉后,花粉萌发和花粉管生长均受到明显抑制,花粉管发生弯曲,膨大,甚至出现多个花粉管萌发等现象。花粉管壁的成分也发生变化。荧光显微镜下观察,正常生长的花粉管纤维素和胼胝质在细胞壁上呈均匀分布,其中胼胝质在顶端微弱分布。处理后,花粉管顶端纤维素荧光较对照明亮,胼胝质并未在花粉管顶端积累。运用免疫荧光标记技术及激光共聚焦显微镜观察,酸性果胶质、酯化果胶和阿拉伯半乳聚糖蛋白均存在于正常生长的花粉管壁上。经过处理后,酸性果胶质和酯化果胶质在花粉管顶端积累,阿拉伯半乳聚糖蛋白明显增加。通过显微红外光谱(FTIR)技术分析,进一步验证了上述处理导致花粉管壁酸性果胶质和酯化果胶以及壁蛋白含量的增加。上述结果表明,LaCl3改变了细胞壁多糖和蛋白分布、含量以及细胞壁的延展性,从而引起花粉管生长的异常。
In this study, the effect of LaCl3 , calcium channel blocker, on apple pollen tube and wall components was investigated. Results illustrated that LaCl3 inhibited the pollen germination and tube growth, resulting in abnormal pollen tube. The cytological results indicated that the pollen tube wall was mainly composed of cellulose, callose, pectins and arabinogalactan proteins （AGPs）. Acidic pectin and esterified pectin were uniformly distributed on the control tube wall, but on the LaCl3？treated tube wall, acidic pectin and esterified pectin accumulated in the apex. AGPs were distributed mainly on the basal part of the tube in control pollen, but LaCl3？treatment made AGPs accumulated on the whole tube. FTIR microspectroscopy illustrated that LaCl3 induced obvious changes in the composition of pollen tube walls. There was an increase in acid pectin and proteins, which was in agreement with the result of immunolabeling with JIM5 and LM2. In a word, the results suggested that laCl3 induced the changes in the composition of the tube wall, which further ceased the growth of pollen tube.
出处
《电子显微学报》
CAS
CSCD
2015年第1期62-66,共5页
Journal of Chinese Electron Microscopy Society
基金
国家自然科学基金资助项目(No.31270719)
北京市属高等学校高层次人才引进与培养计划项目(No.CIT&TCD201304095)
