摘要
目的:观察静压力对人牙周膜干细胞(h PDLSCs)骨向分化能力的影响。方法:体外培养h PDLSCs,并将其随机分为4个组;置于压力加载装置内分别给予0(对照组)、20、100、200 k Pa的静压力刺激,连续加压6 h后分别采用Quantitative RT-PCR和Western-blots法检测h PDLSCs的破骨细胞核因子KB受体活化因子配基(RANKL)及骨保护因子(OPG)的表达。结果:与对照组相比,当压力值为20 k Pa时,RANKL mRNA和蛋白的表达量变化不明显(P>0.05),但OPG的表达量明显升高(P<0.05),RANKL/OPG的比值明显降低(P<0.05);当压力值为100 k Pa时,RANKL mRNA和蛋白的表达量明显升高(P<0.05),但OPG的表达量降低(P<0.05),RANKL/OPG的比值明显升高(P<0.05);当压力值为200 k Pa时,RANKL、OPG mRNA和蛋白的表达量均明显降低(P<0.05),其中以RANKL下降的程度更加明显,RANKL/OPG的比值明显降低(P<0.05)。结论:持续的静压力作用可使h PDLSCs表达OPG和RANKL的水平发生明显变化,并具有力值依赖性。
AIM：To investigate the effect of hydrostatic pressure on the osteogenic differentiation of human periodontal membrane stem cells （hPDLSCs）.METHODS：HPDLSCs were continuously treated with 20,100 and 200 kPa hydrostatic pressure for 6 h.Cells without hydrostatic pressure treatment served as the controls.The expression of receptor activator nuclear factor kB ligand （RANKL）and osteoprotegerin （OPG）was assessed by quantitative real-time PCR and Western blot.RESULTS：The 20 kPa pressure significantly increased OPG expression in HPDLSCs （P〈0.05）,it showed no significant effects on RANKL expression （P 〉0.05）,thus decreased RANKL/OPG ratio （P 〈0.05）.When the pressure increased to 100 kPa,RANKL expression was up-regulated （P 〈0.05）,OPG ex-pression was slightly decreased （P 〉0.05）,leading to increase of RANKL/OPG ratio （P 〈0.05）.200 kPa pressure reduced both RANKL and OPG expression,RANKL showed more reduction （P 〈0.05）,therefore RANKL/OPG ratio was decreased （P 〈0.05）.CONCLUSION：Hydrostatic pressure affect the expression of both RANKL and OPG.
出处
《牙体牙髓牙周病学杂志》
CAS
2015年第3期137-141,165,共6页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金(81171001)
