摘要
该文通过多指标正交实验设计对巴马香猪精液冷冻稀释液中低密度脂蛋白(LDL,因素A)、海藻糖(因素B)和甘油(因素C)等3种保护剂联合使用时的作用进行评价,并分别对3种物质的最佳体积质量分数、摩尔浓度和体积百分比进行优化.结果表明3种保护剂对精子活率(TMS)、质膜完整性(PMI)和彗星率(CR)作用的主次顺序均为A>C>B,对精子顶体完整性(AI)作用的主次顺序为B>A>C;3种保护剂对TMS和PMI均有极显著的保护作用(p<0.01),对CR均无显著性作用(p>0.05);LDL和海藻糖对AI有显著的保护作用(p<0.05),甘油对AI无显性作用(p>0.05);通过因素与指标关系趋势图的绘制,发现由各个指标得到的最佳组合均为A2B3C2,且指标间的相关性均达到了显著水平(p<0.05).因此,用TMS,PMI,AI和CR评价巴马香猪冷冻-解冻后精液质量时,指标间不存在矛盾,用9%(w/v)低密度脂蛋白、200mmol/L海藻糖和2%甘油为巴马香猪精液冷冻稀释液中最优组合.
By a multi-index orthogonal test, the effects of low-density lipoprotein （LDL, factor A）, trehalose （factor B） and glycerol （factor C） used in combination were assessed and analyzed, the mass to vol- ume ratio, molarity, and volume percentage of the three reagents were in the sequence of A〉C〉B on total motile sperm （TMS）, plasma membrane integrity （PMI） and comet rate （CR） and of B〉A〉C on ac rosome integrity （AI）, and the optimum combination was A2B3C2. LDL, trehalose and glycerol all had significant protective effects on TMS and PMI （p〈~0.05）, LDL and trehalose had significant effects on AI （p〈0.05）, and glycerol had no significant effect on AI （p^0.05）. The relativity was significant （p~ 0.05） or highly significant （p〈0.01） of the indexes; the regression equations between TMS and the other three indexes were effective （p^0.01）, and the regression coefficients were all significant （p〈0.05）. It is, therefore, concluded that when TMS, PMI, All and CR are used to assess the quality of frozen-thawed Bama miniature boar semen, no contradiction will exist between the indexes and that 90/00 （w/v） LDL＋200 mmol/L trehalose＋2% glycerol is the optimum combination of freezing diluent of Bama miniature boar semen.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第7期1-7,共7页
Journal of Southwest University(Natural Science Edition)
基金
国家重点基础研究发展计划(973计划)重大科学问题导向项目资助(2011CBA01006)
遵义医学院重点学科建设项目资助(XZXK-20120702)
2013年重庆高校创新团队建设计划项目资助
