摘要
目的:建立一种检测罗库溴铵的电化学发光(ECL)方法。方法:利用正交设计实验对检测体系中三联吡啶钌(Ru(bpy)32+)浓度和磷酸缓冲盐(PBS)浓度及pH值实行搭配实验,并对实验结果进行多元二次回归分析,得到最佳检测条件。结果:Ru(bpy)32+浓度、PBS浓度及pH值的最佳实验条件分别为1.0 mmol/L、102.5 mmol/L和8.0。本方法检测线性范围为0.1至100μg/mL,检测限为0.06μg/mL。结论:该方法快速、简单。可用于罗库溴铵注射液的测定。
Objective： To develop a method for determination of rocuronium by Electrochemiluminescence（ ECL ） in tris （ 2,2 - bipyridyl） ruthenium（ TBR, Rufbpyf32 ＋ ） phosphate buffer salt（PBS）. Method： Based on L16（43} orthonormal design, the value of pH in PBS, the concentrations of TBR and PBS was investigated, the results were analyzed by multivariate quadratic regression. Result： The optimal conditions of rocuronium by ECL was obtained as follow： the concentration of （ Ru （ bpy ） 32 ＋ ） was 1.0 mmol/L, and PBS was 102.5 mmol/L （ pH 8.0）. The linearity range was from 0.1 - 100μg/mL for rocuronium. The detection limit was 0.06/zg/mL. Conclusion： The established method was fast and simple, suitable for the determination rocuronium for injection.
出处
《激光杂志》
CAS
CSCD
北大核心
2013年第2期97-98,共2页
Laser Journal
关键词
罗库溴铵
电化学发光
正交设计
rocuronium
electrochemiluminescence
orthonormal design
