摘要
为筛选能有效控制副猪嗜血杆菌病的中药,本研究采用肉汤二倍稀释法和琼脂平板培养计数法探讨黄连等16种中药提取物对副猪嗜血杆菌的体外抗菌作用。结果显示,黄连和黄柏对副猪嗜血杆菌的抗菌效果最好,其最小抑菌浓度(minimum inhibitory concentration,MIC)和最小杀菌浓度(minimum bactericidal concentration,MBC)均为0.98g/L;黄芩、虎杖、秦皮的MIC为1.95g/L,抗菌效果较好;其次是蒲公英、连翘等10味中药,其MIC范围为3.90～62.50g/L;而白鲜皮和紫花地丁的抗菌效果相对较差,MIC为125.00g/L。
To get traditional Chinese medicine which could control Haemophilus parasuis effectively,the antimicrobial activitiy in vitro of extracts from 16 kinds of traditional Chinese medicine a- gainst Haemophilus parasuis were investigated with methods of serial two-fold Broth dilution method and agar plate counting method. The results showed that Rizoma Coptidis and Cortex Phellodendr Chinensis had the best antimicrobial activity agianst Haemophilus parasuis, the minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） was 0. 98 g/L. Meanwhile, MIC of Radix Scutellariae ,Rhizoma Polygoni Cuspidati ,Cortex Fraxini was 1.95 g/L, the antibacterial effect was better. Other ten kinds of traditional Chinese medicine extracts such as Herba Taraxaci ,Fructus Forsythiae and so on, their MIC range was 3. 90-62. 50 g/L. The antibacterial effect of Cortex Dictamni and Herba Violae was poorer,their MIC was 125.00 g/L.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2014年第8期1324-1327,共4页
Chinese Journal of Veterinary Science
基金
"十二五"农村领域国家科技计划资助项目:现代中兽药研究与新药创制(2011BAD34B03-4)
关键词
中药提取物
副猪嗜血杆菌
抗菌活性
MIC
MBC
traditional Chinese medicine extracts
Haemophilus parasuis
antimicrobial activity
MIC
MBC
