摘要
根据14-3-3蛋白的保守性及菜豆基因组信息,从普通菜豆品种东北小油豆中克隆了一个14-3-3蛋白基因Pv GF14h。生物信息学分析表明,该基因编码的蛋白以α螺旋为主导结构,具有典型的14-3-3蛋白结构域,主要分布在细胞质与过氧化体中,具有磷酸化位点;序列比对及进化树分析表明,它与大豆14-3-3蛋白SGF14h有较高的同源性(可达98.1%);进一步利用实时定量RT-PCR分析Pv GF14h响应冷胁迫的表达模式,结果表明该基因的表达随着冷胁迫处理时间的增长而增加,因此推测Pv GF14h可能参与了冷胁迫的信号转导过程。这些关于菜豆14-3-3蛋白基因的基本信息为进一步研究其功能尤其在响应低温胁迫方面奠定了基础。
A 14-3-3 gene, Pv GF14 h, was cloned from Phaseolus vulgaris L. based on the conservation of 14-3-3 protein and the genome sequence of common bean. Bioinformatics analysis indicated that the protein encoded by Pv GF14 h was characterized with alpha-helix predominant structure, typical 14-3-3 domain, phosphorylation sites,and localized at cytoplasm and peroxisome. Sequence alignment and phylogenetic analysis showed that Pv GF14 h had the highest homology with SGF14 h from soybean, and the identity was up to 98.1%. Furthermore, real-time PCR analysis was conducted to examine its expression pattern in response to cold stress. The result showed that the transcriptional level was promoted by cold treatment, suggesting that Pv GF14 h might be involved in the signaling transduction during cold stress. The findings provided some preliminary and important information about the 14-3-3in common bean, which would facilitate to develop further study for understanding its functions, especially in response to cold stress.
出处
《广东农业科学》
CAS
2015年第4期106-112,共7页
Guangdong Agricultural Sciences
基金
国家自然科学基金(31300253)
大学生创新创业训练计划项目(2013A82283)
