摘要
从采油厂受石油污染的土壤中筛选和驯化两株耐低温石油降解菌株JA和JB,以长庆原油为反应底物,采用响应面法考察p H、原油初始浓度和生物接种量对原油降解率的影响,并优化降解条件,在优化条件下进行降解动力学实验。结果表明,单因素对原油降解率的影响顺序为:p H>原油初始浓度>生物接种量。p H和生物接种量的交互影响对原油降解率的影响显著,根据响应面模型计算得到的最佳降解条件为:p H=7.15,原油初始浓度3 387 mg·L-1,生物接种量75 m L·L-1。3天的原油降解率最高达65.76%,低温复配菌株降解过程符合一级动力学模型。
JA and JB, two strains of oil degradation bacteria with low temperature resistance, were screened and domesticated from soil contaminated by oil in oil production plant. Using Changqing crude oil as the substrate, the effects of pH values, initial concentration of crude oil and biological inoculation amount on the degradation rate of crude oil were investigated by the response surface method. Then by optimizing the degradation conditions, the degradation kinetics experiment was carried out. The results showed that the influence degree of single factor on crude oil degradation in the sequence was as follows ： pH value 〉 crude oil initial concentration 〉 biological between pH value and biological inoculation amount on inoculation amount; the influence of the interaction crude oil degradation rate was extremely remarkable. According to the response surface model, the best degradation condition was pH = 7.15, crude oil initial concentration 3 387 mg · L^-1, and biological inoculation amount 75 mL · L^-1. The highest crude oil degradation rate of 65.76% was attained for 3 days. The degradation process of compound bacteria with low temperature resistance followed the first-order kinetic model.
出处
《工业催化》
CAS
2015年第3期244-248,共5页
Industrial Catalysis
关键词
化学动力学
石油降解菌株
低温复配菌株
响应面分析
chemical kihetics
oil degradatioh bacteria
low-temperature compouhd bacteria
respohse surface ahalysis
