摘要
目的:采用微波辅助萃取提取龙眼核中的多酚,并测定其抗氧化活性。方法:采用微波辅助萃取提取龙眼核中的多酚,采用Folin-Ciocalteus法测定多酚含量并计算提取率;测定多酚的总抗氧化能力、对羟自由基和DPPH自由基的体外抗氧化能力。结果:微波提取率是13.21±0.37%,龙眼核多酚的总抗氧化能力为30.03±3.72U/mg、羟自由基清除IC50和DPPH自由基清除IC50分别为0.00738±0.000172mg/mL、0.00787±0.000372mg/ml;在低浓度范围内DPPH自由基清除率优于维生素C(VC)。结论:龙眼核多酚能有效地清除羟自由基和DPPH自由基,在一定范围内清除自由基能力和浓度成线性关系,是优良的天然自由基清除剂。
Polyphenols were extracted from longan seeds by using microwave-assisted extraction.The content of polyphenols was detemined by the Folin-Ciocalteus method,and the extracted rates were calculated.The antioxidant activity of extracts were evaluated by total reducing power,superoxide anion radical and DPPH free radical scavenging activities.The extracted rate of polyphenol was 13.21±0.37 %,and IC50 of Hydroxyl radicals and DPPH radical were 0.00738±0.000172 mg/m L and 0.00787±0.000372 mg/m L respectively.Polyphenols from longan seeds were excellent natural free radical scavenger,with linear relationship between concentrations and free radical scavenging abilities.
出处
《广东化工》
CAS
2015年第5期176-177,共2页
Guangdong Chemical Industry
基金
2012~2013年度广州医科大学大学生科技创新项目2013A0008
<2014年国家级大学生创新创业训练计划项目>编号:201410570014
关键词
微波萃取
多酚
微波
抗氧化
microwave-assisted extraction
ployphenol
antioxidant
