摘要
将类转录激活因子效应物核酸酶(Transcription activator-like effector nuclease,TALEN)打靶技术运用到水稻叶绿体转化体系中,有可能提高外源基因在水稻叶绿体基因组中的整合效率。根据叶绿体表达载体上的同源重组序列,构建了水稻叶绿体TALEN载体,经分子检测和DNA测序分析证明载体构建成功;将该载体转化大肠杆菌,载体上携带的EGFP、hpt和Fok基因在大肠杆菌中得到成功表达,说明构建的水稻叶绿体TALEN载体能够在原核系统中有效表达。
The TALEN targeting technology was applied to rice chloroplast transformation system to improve the integration efficiency of foreign genes in rice chloroplast genome. A TALEN vector for rice chloroplast was constructed according to the homologous recombination sequence of chloroplast expression vector. Molecular analysis and DNA sequencing showed that the vector was successfully constructed. The genes of EGFP,hpt and Fok were expressed in E. coli transformed with the TALEN vector,indicating that the TALEN vector for rice chloroplast was effectively expressed in E. coli.
出处
《杂交水稻》
CSCD
北大核心
2015年第2期63-68,共6页
Hybrid Rice
基金
国家"863"计划(2014AA10A6032011AA10A101)
国家转基因作物新品种培育重大专项(2011ZX08001-001
2011ZX08001-004)
湖南省科技计划重点项目(2014FJ2014)
湖南工业大学自然科学研究计划项目(2014HZX02)
国家自然科学基金(3401456)
杂交水稻国家重点实验室(湖南杂交水稻研究中心)开放课题基金(2014KF04)
