摘要
目的检测肝纤维化大鼠的阴虚证表征,观察滋补肝肾中药一贯煎对阴虚证表征的影响。方法将48只雄性Sprague-Dawlay大鼠随机分为4组,正常对照组、模型组、阳性对照组、一贯煎组,每组12只。除正常对照组外,均建立CCl4致肝纤维化大鼠模型,在CCl4停止注射后3 d,一贯煎组每只动物给予相应浓度的一贯煎浓缩液2 m L/200 g灌胃,1次/d。阳性对照组给予秋水仙碱灌胃,0.25 mg/kg,1次/d。正常对照组和模型组按相同方法灌服等量生理盐水。4周后,检测各组大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)等肝功能指标;通过HE染色评价肝脏炎症活动度(G)和纤维化程度(S);检测大鼠体重、舌面干湿度、舌面温度、舌微循环血流速度、饮水、大小便等阴虚证表征。结果 1一贯煎在改善肝功能、减轻炎症、抗肝纤维化方面作用较好:一贯煎组ALT[(41.66±3.72)U]较模型组[(61.00±0.00)U]降低,差异有统计学意义(P<0.05);一贯煎组AST[(146.03±46.41)U]较模型组[(218.90±12.56)U]降低,差异有统计学意义(P<0.05);一贯煎组炎症活动度[(1.00±0.00)]较模型组[(3.00±0.00)]降低,差异有统计学意义(P<0.05);一贯煎组纤维化程度[(1.00±0.00)]较模型组[(2.00±0.00)]降低,差异有统计学意义(P<0.05)。2肝纤维化大鼠具有阴虚证的表征,一贯煎能够改善阴虚证的症状。体重:模型组的体重[(380.66±37.29)g]较正常对照组[(507.00±17.69)g]减少(P<0.05);一贯煎组体重[(473.66±13.86)g]较模型组增高(P<0.05)。舌面干湿度:模型组的舌面干湿度[(0.000 36±0.000 28)g]较正常对照组[(0.007 32±0.001 26)g]减少(P<0.05);一贯煎组舌面干湿度[(0.012 83±0.005 85)g]较模型组增高(P<0.05)。舌面温度:模型组的舌温度[(34.48±1.24)℃]较正常对照组[(30.63±0.80)℃]增高(P<0.05);一贯煎组舌温度[(31.40±2.10)℃]较模型组降低(P<0.05)。舌血流速度:模型组的舌血流速度[(69.78±21.44)AU]较正常
Objective To detect the Yin deficiency symptoms of hepatic fibrosis in rats, observe the effect of traditional Chinese medicine Yiguanjian on characterization of Yin deficiency syndrome. Methods 48 male Sprague-Dawlay rats were randomly divided into 4 groups： normal group, model group, positive control group, Yiguanjian group, each group had 12 rats. Except the normal group, hepatic fibrosis model induced by CCl4 was established. After 3 days when the CCl4 injection was stopped, each rat in Yiguanjian group was given Yiguanjian（2 m L/200 g）by intragastric administration, 1 time/d. Positive control group was given Colchicine（0.25 mg/kg） by intragastric administration, 1 time/d. The normal group and model group were fed with same volume of saline daily. After 4 weeks, serum levels of aspartate aminotransferase（AST） and alanine aminotransferase（ALT） were measured by routine methods; the HE staining was used to evaluate the semi-quantitative scoring of liver inflammation（G） and liver fibrosis（S）; the features of Yin deficiency syndrome like body weight, tongue humidity,temperature of tongue, tongue microcirculation blood flow velocity, drinking, stool and urine were detected. Results1 Yiguanjian had good effect in improving liver function, reduce inflammation, anti-liver fibrosis. Compared with the model group [（61.00±0.00） U], ALT was significantly declined in Yiguanjian group [（41.66±3.72） U], with statistically significant difference（P〈0.05）. Compared with the model group [（218.90±12.56） U], AST was significantly declined in Yiguanjian group [（146.03±46.41） U], with statistically significant difference（P〈0.05）. Compared with the model group[（3.00±0.00）], the semi-quantitative scoring of liver inflammation was significantly declined in Yiguanjian group [（1.00±0.00）], with statistically significant difference（P〈0.05）. Compared with the model group [（2.00±0.00）], the semi-quantitative scoring of fibrosis was significantly declined in Yigua
出处
《中国医药导报》
CAS
2015年第7期7-10,18,共5页
China Medical Herald
基金
国家自然科学基金资助项目(编号81273668)
关键词
肝纤维化
阴虚证
一贯煎
Hepatic fibrosis
Yin deficiency syndrome
Yiguanjian
