摘要
目的探讨siRNA干扰Runx3对人肝癌Hep G2细胞增殖、凋亡和侵袭能力的影响。方法以人肝癌Hep G2细胞为研究对象,构建Runx3-siRNA,转染入细胞。将细胞分为对照组、脂质体组、NC-siRNA组和Runx3-siRNA组。用RT-PCR法检测Runx3 mRNA表达,用Western blot检测Runx3蛋白表达。用MTT法、流式细胞法和Transwell小室法观察Runx3对Hep G2细胞增殖、凋亡和侵袭的影响。结果 Runx3-siRNA可抑制Hep G2细胞的增殖(P<0.05);Runx3-siRNA可促进Hep G2细胞凋亡(P<0.05);Runx3-siRNA可抑制Hep G2细胞的侵袭(P<0.05)。结论Runx3基因在肝癌的发生发展中发挥一定的促进作用,有可能成为肝癌治疗的新靶点。
Objective To explore the effect of siRNA targeting Runx3 on proliferation,apoptosis,and invasive ability of Hep G2 cells. Methods The Runx3-siRNA was transfected into Hep G2 cells. Hep G2 cells were divided into four groups： control group,liposomes group,NC-siRNA group and Runx3-siRNA group. The expression levels of Runx3 mRNA and Runx3 protein were examined by RT-PCR and Western blotting. MTT assay,flow cytometry and Transwell chamber were used to determine the effect of Runx3 on Hep G2 cells proliferation,apoptosis and migration. Results MTT assay showed that the proliferation of Hep G2 cells was obviously inhibited by Runx3-siRNA. Flow cytometry demonstrated that the apoptosis of Hep G2 cells was promoted by Runx3-siRNA. The invasion of Hep G2 cells was also significantly inhibited by Runx3-siRNA according to Transwell chamber assay. Conclusions Silence Runx3 could effectively inhibit cell proliferation and migration,and promote apoptosis in Hep G2 cells. Our study shows that Runx3 gene plays an important role on the occurrence and development in hepatocellular carcinoma. Runx3 is a potential target in the treatment of hepatocellular carcinoma.
出处
《基础医学与临床》
CSCD
2015年第3期383-386,共4页
Basic and Clinical Medicine
