摘要
目的研究血清血管内皮生长因子(VEGF)和骨桥蛋白(OPN)的表达与胃癌浸润、转移等病理分期的相关性,探讨二者联合检测诊断胃癌的临床意义。方法采用酶联免疫吸附试验法(ELISA)检测40例胃癌患者(胃癌组),30例健康体检者(健康组)血清中VEGF、OPN的浓度,并分析其与胃癌患者临床病理因素之间的关系。结果胃癌患者血清VEGF、OPN水平明显高于健康组(P<0.01);胃癌患者血清VEGF和OPN的浓度与胃癌病理分期、肿瘤大小、淋巴结转移、远处转移及分化程度相关(P<0.05);胃癌患者血清OPN浓度与VEGF浓度呈正相关(r=0.5431,P<0.05);VEGF、OPN单独检测诊断胃癌其敏感性分别为67.5%、77.5%,二者联合检测敏感性提高至92.7%。结论血清中VEGF、OPN的表达水平与胃癌的浸润、转移密切相关,VEGF、OPN表达具有正协同作用,两者联合检测能更准确预测胃癌的浸润、转移等病理分期。
Objective Study of serum vascular endothelial growth factor （VEGF） and osteopontin （OPN） the relationship between the expression of gastric cancer invasion, metastasis and other pathological staging to study the serum vascular endothelial growth factor （VEGF） and osteopontin （OPN） expression and the correlation with tumor invasion and metastasis, and investigate the clinical significance of combined detec- tion of both. Methods Serum VEGF and OPN concentrations in 40 patients with gastric cancer （ gastric cancer group） and 30 healthy subjects （healthy group） were detected by enzyme-linked immunosorbent assay （ELISA） and the clinical pathological factors relations were analyzed. Results Serum VEGF and OPN levels of patients with gastric cancer were significantly higher than that of the healthy group, the difference was statistical- ly significant （P 〈0.01 ）. Serum OPN and VEGF concentrations were closely related to the clinical staging of gastric cancer, tumor size, lymph node metastasis, distant metastasis and the degree of differentiation （P 〈 0.05 ）. Serum concentrations of OPN in patients with gastric cancer was positively correlated with VEGF con- centrations （r = 0.5431 ,P 〈0.05）. Detection sensitivity of VEGF or OPN alone was 67.5% or 77.5% re- spectively, while combined detection sensitivity increased to 92.7%. Conclusion Serum VEGF and OPN ex- pression levels were closely related to the invasion and metastasis of gastric cancer. VEGF and OPN expression has a positive synergistic effect with eath other. Combined detection of the expression levels of OPN and VEGF can improve the diagnosis of gastric cancer, more accurately.
出处
《湖北科技学院学报（医学版）》
2015年第1期1-3,12,共4页
Journal of Hubei University of Science and Technology(Medical Sciences)
基金
湖北省自然科学基金资助项目(2011CDB316)
咸宁市科技研究与开发项目(2012FFC11601)
关键词
血管内皮生长因子
骨桥蛋白
胃癌
浸润
转移
Vascular endothelial growth factor
Osteopontin
Gastric cancer
Invasion
Metastasis
