摘要
以抗草甘膦油菜基因组DNA为模板,PCR扩增抗草甘膦油菜的5-烯醇式丙酮酸莽草酸-3-磷酸合成酶(EPSPS)基因,构建植物表达载体pBI121-EPSPS,并导入农杆菌进行检测.结果表明:扩增获得EPSPS基因全长1 377bp,共编码459个氨基酸.测序结果表明,其与美国Monsanto公司获得的专利(US5633435)中已知的CDS序列完全一致,表明成功构建了EPSPS植物表达载体,并将其导入农杆菌菌株LBA4404中.
EPSPS gene was amplified from transgenic herbicide glyphosate resistant oil flax.The se-quence was constructed into plant express vector pBI1 2 1-EPSPS,which was transferred into Agrobacterium tumefaciens.The results indicated that the full-length of the amplified EPSPS gene was 1 377 bp,which encoded 459 amino acids.The sequencing results showed the identical CDS sequence as the known one in Monsanto’s patent （US5633435）.The plant expression vector of EPSPS was constructed successfully and transferred into A.tumefaciens LBA4404.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2015年第1期53-57,共5页
Journal of Gansu Agricultural University
基金
国家胡麻产业技术体系项目(CARS-17-GW-02)
国家自然科学基金项目(31260355)
