摘要
亚洲象(Elephas maximus)是我国国家一级保护动物,准确地评估其种群大小和遗传多样性对该物种的保护具有十分重要的意义。本文采用非损伤性取样方法,并结合野外观察数据对尚勇保护区当前亚洲象种群数量进行了评估,同时对该种群的遗传多样性进行了分析。采用7对微卫星引物对185份粪便样品进行扩增,通过基因分型,得到59个独特的基因型。文章采用两种方法评估种群数量,即聚集曲线法和分子标记重捕法,估计尚勇保护区的亚洲象种群数量为76±8头(95%置信区间内为67–99头)。遗传多样性方面,平均等位基因数为3.86,平均观察杂合度为0.52,平均期望杂合度为0.42,平均多态信息含量为0.34,表明尚勇保护区亚洲象种群遗传多样性水平为中等偏低,与勐养种群遗传多样性水平相似。
Asian elephant （Elephas maximus） is a first class protected species in China. Estimation of the population size and genetic variability is crucial to the management and conservation of this species. A ge- netic survey was conducted using multi locus genotyping of noninvasively collected fecal samples of the Asian elephant population in the Shangyong Nature Reserve, Yunnan Province of China. Amplification of 185 fecal DNA samples by 7 microsatellite primers resulted in a total of 59 unique genotypes. The average number of alleles was 3.86, the average observed and expected heterozygosity and polymorphism information content （PIC）, was 0.52, 0.42 and 0.34, respectively. Accumulation curve method and capture mark re-capture were used to evaluate the population size. Results indicated that the population size in Shangyong Nature Reserve was 76 4- 8 （from 67 to 99 individuals, 95% confidence interval）. Overall, a moderate level of genetic variability was observed in the Shangyong population, which was similar to a population in the Mengyang region.
出处
《生物多样性》
CAS
CSCD
北大核心
2015年第2期202-209,共8页
Biodiversity Science
基金
国家自然科学基金(31172108)
国家985重点建设项目北京师范大学生物多样性科学与濒危物种保育科技创新平台及美国内政部鱼和野生动物管理局亚洲象保护基金(98210-8-G565)
