摘要
目的：建立复方罗布麻颗粒中木犀草苷的含量测定方法。方法采用高效液相色谱法测定复方罗布麻颗粒菊花中木犀草苷的含量,采用Phenomenex luna C18色谱柱（150 mm×4.6 mm,5μm）,流动相为乙腈-0.5%冰醋酸（14∶86）,流速为0.8 mL/min,检测波长为348 nm。结果木犀草苷在0.0319～0.7963μg范围内呈良好的线性关系（r＝0.9996）,平均回收率为100.85%（n＝6）。结论本试验所建立的方法专属性强、简便,提高了制剂的质量标准,增强了本品的可控性。
Objective To establish determination method of luteolin-7-O-glucoside in Compound Luobuma Granule. Methods Phenomenex luna C18 column （4.6 mm×150 mm, 5 μm） was used;aceto-0.5% acetic acid （14∶86） was set as the mobile phase at flow rate of 0.8 mL/min;the detection wavelength was 348 nm. Results The linear range of luteolin-7-O-glucoside was in the range of 0.031 9-0.796 3 μg （r=0.999 6）, and the average recovery was 100.85% （n=6）. Conclusion The method is specific, simple, and can improve quality standard and increase the controllability of Compound Luobuma Granule.
出处
《中国中医药信息杂志》
CAS
CSCD
2015年第4期91-93,共3页
Chinese Journal of Information on Traditional Chinese Medicine
