摘要
为了检验已记录的3种裸腹溞（发头裸腹溞（Moina irrasa）、短型裸腹溞（Moina brachiata）、微型裸腹溞（Moina micrura））的系统分类,用试剂盒法分别提取3种裸腹溞的基因组DNA.利用特异性引物,通过PCR扩增了3种裸腹溞的16S r DNA部分序列,并与来自Gen Bank中每个种类相似度较高的裸腹溞属序列进行分析.结果表明,3种裸腹溞的平均种间相似度为88.7%,碱基中A＋T含量均明显高于G＋C含量.本研究的发头裸腹溞的16S r DNA序列与Gen Bank所下载的多刺裸腹溞（Moina macrocopa）的16S r DNA序列相似度为99%,遗传距离（K2P双参考模型）为0.5%,属种内范围;两个地区的短型裸腹溞测得的16S r DNA序列与Gen Bank下载的欧洲短型裸腹溞的16S r DNA序列序列相似度相对较低（88%~90%）,遗传距离较大（13.2%~13.5%左右）,已达到属内种间分化水平.基于16S r DNA构建的NJ树和贝叶斯树也支持以上结论.结果表明,本研究的发头裸腹溞可能为多刺裸腹溞,本研究用的短型裸腹溞与Gen Bank下载的欧洲短型裸腹溞已经达到种间分化的标准.由于缺乏物种形态资料和其他分子标记的对比,3种裸腹溞的分类地位还需进行更深入的探讨.
In order to examine the systematic classification of three Moina species, M. irrasa, M. brachiata and M. micrura recor- ded previously, genomic DNA of three Moina species were extracted through the kit method. PCR was used to amplify partial se- quences of 16S rDNA of three Moina species by employing specific primers, and same sequence with higher matching rate of each Moina species from the GenBank were analysed. Based on 16S rDNA, the average interspecific similarity rate among three Moina species was 88.7% , and the content of A ＋ T was obviously higher than that of G ＋ C. The similarity of M. irrasa in this study and M. macrocopa from the GenBank was 99% , and the divergence rate was 0.5% , within a range of same species. The similarity of M. brachiata in this study and the GenBank was relatively low（ 88% -90% ）, and the divergence rate was 13.2% -13.5% , with- out a range of same species. The NJ tree and Bayes tree based on 16S rDNA did also support the above results. The results sugges- ted that M. irrasa in this study and M. macrocopa from the GenBank might be the same species, and M. brachiata from this study and the GenBank had reached the standard of species divergence. Due to the lack of morphology and other genes analysis, the clas- sification status of three Moina species in this study mentioned need to be further investigated.
出处
《湖泊科学》
EI
CAS
CSCD
北大核心
2015年第2期305-310,共6页
Journal of Lake Sciences
基金
国家自然科学基金项目(31370470)资助
