摘要
目的 分析细胞色素C1（CYC1）在神经母细胞瘤（NB）增殖中的作用,探讨神经母细胞瘤增殖的分子机制。方法 首先采用在SH-SY5Y细胞系中慢病毒介导敲减CYC1基因方法,建立神经母细胞瘤CYC1敲减模型,并利用实时定量RT-PCR方法验证敲减效率;随后利用荧光显微镜观察及细胞分析系统连续检测慢病毒感染SH-SY5Y细胞后连续5 d细胞增殖数。结果 慢病毒介导的SH-SY5Y细胞中CYC1基因敲减效率达77.0%±4%,具极显著差异（P〈0.01）;通过细胞分析系统连续检测SH-SY5Y细胞慢病毒感染敲减CYC1后0～5 d细胞数发现细胞增殖速度明显降低,对照组/敲减CYC1组细胞数1～5 d比值分别为（1.00±0.11）、（1.61±0.16）、（1.58±0.17）、（1.97±0.13）、（1.44±0.13）,具极显著差异（P〈0.01）。结论 CYC1基因沉默可抑制神经母细胞瘤细胞SH-SY5Y增殖,CYC1在调节神经母细胞瘤生长增殖方面发挥重要作用。
Objective To investigate the role of CYC1 and the molecular mechanism involved in neuroblastoma proliferation. Methods To establish CYC1-knockdown neuroblastoma cell model, lentivirus introduced CYC1 RNA interference was performed in neuroblastoma SH-SYSY cell line. Reahime-reverse transcription PCR was performed to detect the efficiency of CYC1 RNA interference. The effects on SH-SYSY cells proliferation of CYC1-knockdown were detected by fluorescence microscope and CloneSelect Imager system for 5 days continuously. Results CYC1 was knockdown by 77.0% ±4% in SH-SYSY cells （P 〈 0. 01 ）. CYC1-knockdown significantly reduced the proliferation of SH-SYSY cells via fluorescence microscope and CloneSelect Imager system. The ratio of the control and experimental groups in SH-SYSY cells for 5 days were 1.00±0.11 （P〈0.01）, 1.61 ±0.16 （P〈0.01）, 1.58±0.17 （P〈0.01）, 1.97±0.13 （P〈0.01）, and 1.44±0.13 （P〈0.01） separately. Conclusion The finding indicated that knockdown of CYC1 inhibits the proliferation of neuroblastoma SH-SY5Y cells.
出处
《中国小儿血液与肿瘤杂志》
CAS
2015年第1期10-13,共4页
Journal of China Pediatric Blood and Cancer
基金
国家自然科学基金:儿科疾病及其药物遗传学特点的研究(编号:81342002)
吴阶平医学基金会临床科研专项资助基金:MACC1基因在儿童头颈部肿瘤转移和靶向治疗中的作用(320675012398)
