摘要
[目的]研究宫颈癌Hela细胞中Stathmin-1表达对p53蛋白的影响及对细胞增殖的作用。[方法]构建Stathmin-1 siRNA慢病毒载体,包装重组慢病毒,感染宫颈癌Hela细胞株,沉默Stathmin-1表达为干扰组,采用导入阴性片段的慢病毒感染Hela细胞作为对照组。MTT法检测细胞增殖,蛋白印迹法(Western-blot)检测细胞p53蛋白的表达。[结果 ]感染重组慢病毒及阴性病毒后,Stathmin-1基因干扰组Hela细胞增殖24h后较对照组抑制明显(P<0.05)。与对照组相比,干扰组中p53蛋白表达明显减少(P<0.05)。[结论]宫颈癌Hela细胞中Stathmin-1影响p53蛋白表达,沉默Stathmin-1可抑制细胞增殖。
[Purpose] To study the influences Stathmin-1 on p53 protein expression in Hela cells and the role on cells proliferation. [Methods] Short hairpin RNA（shRNA） targeting Stathmin-1 was designed and lentivirus carried the shRNA infected Hela cells as interference group. Control group cells were infected with lentivirus carried negative fragment. After recombinant ]entivirus infected Hela cells, MTT assay was used to detect the status of proliferation. Western blot was used to ana- lyze p53 protein expression. [Resuhs] With STMN-1 silencing,MTT assay results suggested Hela cells proliferation was suppressed obviously in interference group compared with control group （P〈 0.05）.The p53 protein expression in interference group was decreased （P〈0.05）. [Conclusion] Stathmin-1 expression can influence p53 protein expression in cervical Hela cells. Silencing Stath- min-1 can restrain cervical cancer cells proliferation.
出处
《中国肿瘤》
CAS
2015年第3期246-249,共4页
China Cancer
基金
青岛市科技发展计划(13-1-3-118-jcl)
