摘要
目的通过比较乳腺癌组织中血管内皮生长因子（VEGF）、环氧化酶-2（COX．2）、基质金属蛋白酶-9（MMP-9）三种蛋白的表达与乳腺癌超声重要征象的关系，初步探讨乳腺癌超声征象与分子病理学之间的关系，对乳腺癌患者的早期诊断、治疗及预后提供-定理论依据。方法收集术后经病理证实的89例乳腺癌的标本，所有标本术前均用双盲法由从事多年乳腺超声诊断的医师按照BI—RADS分级法进行分组，同时每个病例均通过免疫组化法检测VEGF、COX-2、MMP-9的水平，分析VEGF、COX-2、MMP-9的表达与超声表现的相关性。结果超声有毛刺征组乳腺癌VEGF、COX-2、MMP-9表达阳性率较无毛刺征组（P〈0．05）明显增高。钙化组与无钙化组VEGF、COX-2、MMP-9表达阳性率无明显差异（P〉0．05）。有血管异常征组VEGF、COX-2、MMP-9表达阳性率较无血管异常征组（P〈0．05）明显增高。淋巴结转移征组VEGF、COX-2、MMP-9表达的阳性率较无淋巴结转移征组（P〈0．05）明显增高。结论VEGF、COX-2、MMP-9的表达水平对乳腺癌超声征象的出现存在影响，并可作为乳腺癌超声征象中恶性征象的生物学基础，同时提示了有此类超声征象的乳腺癌预后差。
Objective By comparing with the relationship between important signs on ultrasound in breast cancer and the protein expression of vascular endothelial growth factor （VEGF）, cyclooxygenase-2 （COX-2） ,metalloprotease-9 （MMP-9） biology, the relationship between molecular pathology and ultrasound features were studied, which provided a certain theoretical basis for the early diagnosis, treatment and prognosis of breast cancer patients. Methods 89 cases of breast lesions were confirmed by ultrasonic examination, opera- tion and pathology. By using double-blind method, ultrasound features were grouped into burr, calcification, abnormal vascular, lymph node by physicians with rich breast diagnosis experience based on BI-RADS classification method. In each case, the expression of VEGF, COX-2, MMP-9 were detected by immunohistochemical SP method, then the relationship between the expression of VEGF, COX-2, MMP-9 and breast ultrasound fea- tures were analyzed. Results positive rate of protein expression of VEGF, COX-2, MMP-9 in burr syndrome group were higher than that of no-burr syndrome group （P 〈0.05 ） , no obvious difference（P 〉0.05 ） between the calcification group and no calcification group, higher in abnormal breast cancer group than that of no- abnormal blood vessels （ P 〈 0.05 ）, higher in lymph node metastasis group than that of no-lymph node metastasis （P 〈 0.05）. Conclusions The high expression of VEGF, COX-2, MMP-9 could have an influence on burr syndrome, abnormal blood vessels, lymph node metastasis in breast cancer ultrasound features, which could beused as a biological basis of malignant signs in uhrasound of I：）reast eancer. And at the same time, it＇ s prompled thal Ihe breast cancer patients with ultrasound features is high in malignant degree and with poor prognosis.
出处
《国际免疫学杂志》
CAS
2015年第2期146-150,共5页
International Journal of Immunology
基金
基金项目：国家自然科学基金（81473687）
山东省自然科学基金（ZR2013HM038）
山东省医药卫生科技发展计划（2011HW084）
