摘要
目的:对蓼科药用植物高山大黄进行显微及分子鉴定研究。方法:采用石蜡切片、粉末制片对高山大黄根及根茎的横切面组织结构和粉末特征进行显微研究,并采用mat K基因序列分析技术对高山大黄进行了分子鉴定研究。结果:高山大黄根横切面中皮层宽,薄壁细胞中可见草酸钙簇晶,木质部宽广,导管径向排列;根茎髓部宽广,无异形维管束存在;粉末中草酸钙簇晶多,棱角大多短钝,导管木质化,多网纹导管,淀粉粒小而多。高山大黄mat K序列全长1 524 bp,在基因上游106、218、222、365、522、615、711、759、801、852处存在十处碱基替换,且在950-955处存在CAAGAA6个碱基插入。结论:本研究获得了高山大黄比较全面的鉴定信息,上述显微特征可作为高山大黄显微鉴定依据,其mat K基因序列分析为高山大黄DNA条形码研究奠定基础。
Objective： To perform a study of the medicinal herbal microscopic and molecular identification of Rheum nobile radix and rhizome. Methods： The cross sections of Rheum nobile radix and rhizome were obtained by using paraffin sectioning,and temporary glycerol tablets with chloral hydrate were used to identify the microscopic structure of powder. The mat K gene sequence analysis technique was used for molecular identification of Rheum nobile. Results： In the cross-section of radix,both the cortex and xylem were wide with vessels arranging radially and massive calcium oxalate crystal visible in parenchyma cells. Pith part was broad without any abnormal vascular bundle. Powder contained a mass of calcium oxalate crystals whose edges were mostly short blunt.There were lignified vessels and small starch grain as well. The length of mat K gene sequence of Rheum nobile was 1 524 bp. Upstream of mat K sequence,there were ten base-pair substitutions in 106,218,222,365,522,615,711,759,801,852,and 6 bases CAAGAA inserted in 950-955. Conclusion： The research tends to contain relatively comprehensive identification information of Rheum nobile. These microscopic features can be used as evidence for identification of Rheum nobile radix and rhizome,and the mat K gene sequence analysis helps lay a foundation for Rheum nobile DNA barcoding research.
出处
《世界中医药》
CAS
2015年第2期261-264,共4页
World Chinese Medicine
基金
北京中医药大学自主选题(藏医药类)课题资助项目(编号:2013-zyy-02)
关键词
高山大黄
显微鉴定
分子鉴定
Rheum nobile
Microscopic identification
Molecular identification
