摘要
目的探讨铜绿假单胞菌甘露糖敏感血凝素(PA-MSHA)对脓毒症致急性肺损伤的影响及可能机制。方法以盲肠结扎穿孔法(CLP)复制大鼠脓毒症模型。按随机数字法将120只健康SD大鼠分为假手术对照组(SC组)、脓毒症组(CLP组)、术前治疗组(Pre T组)和术后治疗组(Pos T组),每组各30只。于制模后12、24、36 h分别采集外周血测定血清肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、IL-10及转化生长因子β(TGF-β)浓度并计算肺损伤病理评分。采用逆转录聚合酶链反应(RT-PCR)方法检测肺组织Toll样受体4(TLR4)m RNA表达,并作肺组织TLR4 m RNA表达与病理学评分的Pearson相关性分析。结果 SC组各时间点血清细胞因子浓度无明显变化(F=0.04、0.09、1.25、1.77,P均>0.05)。与CLP组和Pos T组相比,各时间点Pre T组的血清TNF-α、IL-6浓度明显较低,而IL-10、TGF-β浓度明显较高(P均<0.05),而Pos T组仅在术后36 h的IL-10高于CLP组(P<0.05)。四组大鼠间的肺组织TLR4 m RNA表达水平及四组间各时间点肺组织病理学评分比较,差异均有统计学意义(F=66.01、20.03、71.21、69.02,P均<0.05),SC组及Pre T组明显优于CLP组和Pos T组(P均<0.05)。同时,肺组织TLR4 m RNA表达与病理学评分存在明显的相关性(r=0.732,P<0.05)。结论术前给予PA-MSHA可减轻脓毒症大鼠肺病理损伤,其机制可能与调节外周血单核细胞TLR4表达水平、调控细胞因子平衡、减轻炎症级联放大效应有关。
Objective To investigate the effects and possible mechanism of pseudomonas aeruginosa mannose-sensitive hemagglutinin （PA-MSHA） on acute lung injury in septic rats. Methods Rats septic model was reproduced by cecal ligation puncture （CLP）. Totally 120 healthy SD rats were randomly divided into the sham operation group （SC group）, sepsis group （CLP group）, PA-MSHA treatment before CLP （PreT group）, PA-MSHA treatment after CLP （PosT group）, 30 rats in each group. Rats in each group were sacrificed respectively at 12, 24, 36 hafter operation, and serum tumor necrosis-a（TNF-c0, interleukin-6 （IL-6）, IL-10 and transforming growth factor-β（TGF-β） were examined. The lung tissue samples were collected to calculate the pulmonary pathological scores and determine the expression of TLR4 mRNA by reverse transcription-polymerase chain reaction. Pearson＇s method was employed to analyze the correlation between the expression of TLR4 mRNA and the pulmonary pathological scores. Results There were no significant differences in the serum cytokine concentrations at each time point （F= 0.04, 0.09, 1.25, 1.77; all P 〉 0.05）. In the PreT group, the levels of TNF-a and IL-6 were lower, IL-10 and TGF-13 were higher at each time point than those in the CLP and PosT groups （all P 〈0.05）, and IL-10 in the PosT group only at 36 h was higher than those in the CLP group （P〈 0.05）. The expressions of TLR4 mRNA and lung injury pathological score at each time point all showed significant differences among the four groups （F= 66.01, 20.03, 71.21, 69.02; all P〈 0.05）, which were better in the SC and PreT groups than those in the CLP and PosT groups （all P〈0.05）. Meanwhile, the expression of TLR4 mRNA was correlated with the pulmonary pathological scores （r = 0.732, P〈 0.05）. Conclusions The pathological injury in lung was milder when the septic rat was treated with PA-MSHA before CLP. The mechanism may be related to the adjusted levels of TLR4 expression in peripheral blood m
出处
《中华危重症医学杂志（电子版）》
CAS
2015年第1期3-7,共5页
Chinese Journal of Critical Care Medicine:Electronic Edition
基金
上海市浦东新区卫生系统学科带头人培养计划项目(PWRd2013-11)
