摘要
Pituitary adenylate cyclase activating polypeptide （PACAP） has a wide range of biological functions. We cloned the full-length cDNAs encoding PACAP and PACAP-related peptide （PRP） from the brain of largemouth bass （Micropterus salmoides） and used real-time quantitative PCR to detect PRP- PACAP mRNA expression. The PRP-PACAP cDNA has two variants expressed via alternative splicing： a long form, which encodes both PRP and PACAP, and a short form, which encodes only PACAR Sequence analysis results are consistent with a higher conservation of PACAP than PRP peptide sequences. The expression of PACAP-Iong and PACAP-short transcripts was highest in the forebrain, followed by the medulla, midbrain, pituitary, stomach, cerebellum, intestine, and kidney; however, these transcripts were either absent or were weakly expressed in the muscle, spleen, gill, heart, fatty tissue, and liver. The level of PACAP-short transcript expression was significantly higher than expression of the long transcript in the forebrain, cerebella, pituitary and intestine, but lower than that of the long transcript in the stomach. PA CAP- long and PACAP-short transcripts were first detected at the blastula stage of embryogenesis, and the level of expression increased markedly between the muscular contraction stage and 3 d post hatch （dph）. The expression of PACAP-long and PACAP-short transcripts decreased significantly in the brain following 4 d fasting compared with the control diet group. The down-regulation effect was enhanced as fasting continued. Conversely, expression levels increased significantly after 3 d of re-feeding. Our results suggest that PRP- PA CAP acts as an important factor in appetite regulation in largemouth bass.
Pituitary adenylate cyclase activating polypeptide(PACAP) has a wide range of biological functions. We cloned the full-length c DNAs encoding PACAP and PACAP-related peptide(PRP) from the brain of largemouth bass(Micropterus salmoides) and used real-time quantitative PCR to detect PRP- PACAP m RNA expression. The PRP- PACAP cDNA has two variants expressed via alternative splicing: a long form,which encodes both PRP and PACAP,and a short form,which encodes only PACAP. Sequence analysis results are consistent with a higher conservation of PACAP than PRP peptide sequences. The expression of P ACAP-long and PACAP- short transcripts was highest in the forebrain,followed by the medulla,midbrain,pituitary,stomach,cerebellum,intestine,and kidney; however,these transcripts were either absent or were weakly expressed in the muscle,spleen,gill,heart,fatty tissue,and liver. The level of PACAP-short transcript expression was significantly higher than expression of the long transcript in the forebrain,cerebella,pituitary and intestine,but lower than that of the long transcript in the stomach. P ACAP-long and PACAP-short transcripts were first detected at the blastula stage of embryogenesis,and the level of expression increased markedly between the muscular contraction stage and 3 d post hatch(dph). The expression of PACAP- long and PACAP- short transcripts decreased significantly in the brain following 4 d fasting compared with the control diet group. The down-regulation effect was enhanced as fasting continued. Conversely,expression levels increased significantly after 3d of re-feeding. Our results suggest that PRP- PACAP acts as an important factor in appetite regulation in largemouth bass.
基金
Supported by the National Natural Science Foundation of China(No.31201985)
the National Key Technology R&D Program of China(No.2012BAD26B03)
