摘要
目的:通过观察艾灸对小鼠运动疲劳后心肌细胞凋亡相关因子的影响及组蛋白乙酰化酶p300(histone acetyltransferases p300,p300)、CREB结合蛋白(CREB biding protein,CBP)与细胞凋亡的关系,探讨p300、CBP在艾灸抗心肌细胞凋亡中的作用。方法:清洁级雄性昆明种小鼠60只随机分为对照组、运动组、麦粒灸组,每组20只。各组相同环境饲养,对照组不运动,不施灸;运动组及麦粒灸组进行无负重游泳训练,训练时间由每天30 min逐步延长至90 min,持续21d。麦粒灸组于每天游泳后1h取"足三里""关元"进行麦粒灸治疗,每穴灸5壮,每日1次,共21d。末次游泳结束24h后,取各组小鼠心脏采用免疫组化法检测心肌细胞凋亡因子Fas(factor associated suicide,Fas)、B淋巴细胞瘤-2基因(B cell lymphoma/lewkmia-2,Bcl-2)及p300、CBP的表达。结果:与对照组比较,运动组小鼠心肌细胞凋亡率显著升高(P<0.01),视野内可见密集分布的凋亡小体,且着色较深,心肌细胞中Fas蛋白的表达显著增加(P<0.01),Bcl-2、p300蛋白、CBP表达减弱(均P<0.01);麦粒灸组可见均匀分布的凋亡小体,但着色较浅。与运动组比较,麦粒灸组小鼠心肌细胞凋亡率下降(P<0.05),小鼠Fas蛋白表达降低(P<0.05),心肌细胞中Bcl-2、p300蛋白、CBP表达增强(均P<0.05)。结论:艾灸可以通过促进小鼠疲劳运动后心肌细胞中p300、CBP的表达,抑制细胞凋亡过程的启动,从而减少大运动量后的小鼠心肌细胞的凋亡,保护心脏功能。
Objective To observe the effects of moxibustion on factors related with apoptosis of myocardial cells after sports fatigue in mice as well as the relationship among histone acetyhransferases p300 （p300）, CREB binding protein （CBP） and cell apoptosis to discuss the role of p300 and CBP in moxibustion against apoptosis of myocardial cells. Methods Sixty clean grade mate Kunming mice were randomly divided into a control group, a sport group and a moxibustion group, 20 cases in each one. Mice in all group received identical feeding environ- ment. Mice in the control group did not received sport nor moxibustion; mice in the sport group and moxibustion group received non-weight swimming training which lasted from 30 min per day to 90 min per day gradually for 21 days; 1 h after swimming training, mice in the moxibustion group received moxibustion with seed-sized moxa cone at ＂Zusanli＂ （ST 36） and ＂Guanyuan＂ （CV 4）, 5 cones at each acupoint, once a day for 21 days. 24 h after the final swimming training, cardiac muscle tissue was collected to test factor associated suicide （Fas）, B cell lym- phoma/lewkmia-2 （Bcl-2） by immunohistochemical method and expression of p300 and CBP. Results Compared with the control group, the apoptosis rate of myocardial cells in the sport group was significantly increased （P〈 0.01）, and apoptosis body with dense distribution and deep coloring can be seen in the field of microscope; the ex- pression of Fas protein was significantly increased （P〈0.01）, and expression of Bcl-2, p300 and CBP was reduced （all P〈0.01）. The equally distributed apoptosis body with slight coloring was seen in the moxibustion group. Compared with the sport group, the apoptosis rate of myocardial cells in the moxibustion group was significantly reduced （P〈0.05）; the expression of Fas protein was significantly reduced （P〈0.05）, and expression of Bcl-2, p300 and CBP was increased （all P〈0.05）. Conclusion Moxibustion could promote the expression of p300 a
出处
《中国针灸》
CAS
CSCD
北大核心
2015年第3期257-263,共7页
Chinese Acupuncture & Moxibustion
基金
江苏省高校优势学科建设工程资助项目:ysxk-2010
江苏省普通高校研究生科研创新计划资助项目:CXLX12_0576
江苏省高校自然科学基金研究面上资助项目:13KJB360008
关键词
运动疲劳
麦粒灸
心肌细胞凋亡
组蛋白乙酰化酶p300
CREB结合蛋白
sports fatigue
moxibustion with seed-sized moxa conel myocardial cell apoptosis~ histone acetyla-tion enzyme p300
CREB binding protein
