摘要
目的:研究细胞交互作用对细胞多能性的调控作用。方法:建立牙乳头(DPCs)和牙囊细胞(DFCs)体外共培养模型,二者单独培养的细胞为对照组。采用细胞计数、β-gal染色检测细胞生长和衰老情况;免疫荧光染色检测共培养条件下多能性相关因子Oct-4、Sox2和c-Myc的表达变化;通过ALP活性测试检测矿化诱导后的DPCs和DFCs的矿化能力。结果:共培养组的DPCs和DFCs的细胞数明显高于对照组(P<0.05);培养至第7代时,共培养组的DPCs、DFCs中与衰老相关的SA-b-gal表达较对照组明显减弱(P<0.05);Oct-4、Sox2和c-Myc在共培养组DPCs和DFCs中的表达明显强于对照组;共培养组的DPCs和DFCs经矿化诱导14、21 d后,其ALP活性均显著高于对照组(P<0.05)。结论:DPCs和DFCs可通过体外交互作用抑制细胞衰老、促进细胞的多能性相关因子的表达、提高细胞的矿化能力。
AIM： To investigate the effect of cell-cell interaction of dental papilla cells（ DPCs） and dental follicle cells（ DFCs） on the pluripotency of the cells. METHODS： An in vitro cell-cell co-culture system of DPCs and DFCs was established. Cell growth,cell senescence and the expression of Oct-4,Sox2 and c-Myc were investigated by cell counting,β- gal staining and immunohistochemical staining. The odontogenic differentiation capability of DPCs and DFCs was evaluated by ALP activity assay after 0,7,14,and 21 d of osteogenic induction. RESULTS：Under co-culture condition,cell growth of DPCs and DFCs was promoted,and cell senescence of DPCs and DFCs was inhibited. Expression of Oct-4,Sox2 and c-Myc was significantly elevated in both cells on day 5 after coculture. ALP activity was significantly upregulated in both cells after 14 d and 21 d of odontogenic induction. CONCLUSION： Optimized microenvironment with cell-cell communication may enhance the pluripotency potential of DPCs and DFCs.
出处
《牙体牙髓牙周病学杂志》
CAS
2015年第2期63-67,共5页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助项目(81400499)
高等学校博士学科点专项科研基金(20120171120070)
广东省医学科研基金(B2012141)
中央高校基本科研业务费专项资金(11ykpy51)
关键词
牙乳头细胞
牙囊细胞
微环境
多能性
dental papilla cells（DPCs）
dental follicle cells（DFCs）
microenvironment
pluripotency
