摘要
目的:研究多能性转录因子Nanog在人牙髓细胞连续培养时的表达规律及矿化诱导对其表达量的影响。方法:体外培养人牙髓细胞(DPCs),并对第3代DPCs进行矿化诱导;分别采用实时定量荧光PCR和Westernblot检测P1至P7代以及矿化诱导前后DPCs中Nanog mRNA及蛋白的表达变化情况。数据经统计学分析。结果:在DPCs的体外传代培养中,Nanog mRNA和蛋白表达量均呈下降趋势(P<0.05);矿化诱导后Nanog mRNA和蛋白表达量与对照组相比明显降低(P<0.05)。结论:在牙髓细胞体外连续培养环境下,随着代数增加,Nanog表达量呈下降趋势;矿化诱导可降低Nanog的表达。
AIM： To examine the expression of Nanog in the cultured dental pulp cells （DPCs） and the effect of odontoblastic differentiation induction on Nanog expression. METHODS： DPCs were cultured from human normal tooth pulp by enzymatic digestion, the DPCs from passage 1 to passage 7 were used in the study. DPCs of pas- sage 3 were cultured in the odontoblastic differentiation induction media. Nanog mRNA and protein in the cells were ex- amined by RT-PCR and Western blot respectively. Statistical analysis was performed to compare the differences of Nanog expression in DPCs. RESULTS： Real-time PCR and Western Blot showed that Nanog mRNA and protein levels were decreased with cell passages （ P 〈 0.05 ） and by odontoblastic induction for 14 days （ P 〈 0.05 ）. CONCLU- SION： Culture passage and odontoblastic induction may inhibite Nanog expression in DPCs.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2013年第6期362-365,共4页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金项目(81170953)
广东省自然科学基金(S2011010004931)
关键词
牙髓细胞
NANOG
体外培养
矿化诱导
dental pulp cells
Nanog
in vitro culture
odontoblastic differentiation induction
