摘要
小麦条锈病是小麦生产上最严重的世界性病害之一。小麦品种中梁16具有抗逆性强、高产、抗条锈性强等优良特性。为明确其抗条锈性遗传规律,利用条锈菌小种CYR30对中梁16与感病品种铭贤169及其杂交后代进行苗期抗条锈性遗传分析。结果表明,中梁16对CYR30小种具有良好的抗性,由1对显性基因控制,暂命名为Yr Zhong16。通过分子标记分析,获得了与Yr Zhong16连锁的4个SSR标记Xwmc696、Xgwm644、Xbarc95和Xgwm131。其中与Yr Zhong16最近的侧翼位点为Xgwm644和Xbarc95,其遗传距离分别是2.3和3.5 c M。根据SSR标记的定位结果,将Yr Zhong16定位在小麦染色体7BL上。这些与Yr Zhong16连锁的分子标记为利用中梁16抗条锈病基因进行抗病基因聚合和分子标记辅助育种奠定了基础。
Stripe rust,caused by Puccinia striiformis f. sp. tritici,was one of the most widespread wheat diseases worldwide. Zhongliang 16 was strongly resistant to stripe rust and had excellent biological characteristics. To identify the resistance gene（ s） against stripe rust,Zhongliang 16 was crossed with the susceptible genotype Mingxian 169,and their F1,F2,F2： 3and BC1 progenies were tested with Chinese race CYR30 at seedling stage in greenhouse. Then,the molecular markers linked to the gene（ s） conferring resistance to CYR30 in Zhongliang16 were screen out by SSR techniques. The results showed that the resistance in Zhongliang 16 to race CYR30 was controlled by one dominant gene,temporarily designated as Yrzhong16. Four simple sequence repeat（ SSR） markers located on chromosome arm 7BL,Xwmc696,Xgwm644,Xbarc95 and Xgwm131,were linked to Yr Zhong16 with the nearest flanking markers of Xgwm644 and Xbarc95. Their genetic distances were 2. 3 c M to Xgwm644 and 3. 5 c M to Xbarc95,respectively. The markers could be useful in pyramiding these resistance genes with others to develop wheat cultivars with high-level resistance to stripe rust.
出处
《西南农业学报》
CSCD
北大核心
2015年第1期136-139,共4页
Southwest China Journal of Agricultural Sciences
基金
湖北省重点(优势)学科作物学(长江大学)资助项目
主要农作物涝渍灾害防控关键技术研究与示范(201203032)
国家自然科学基金项目(31000846)
关键词
中梁16
小麦条锈病
抗病基因
遗传分析
分子标记
Zhongliang 162
Wheat stripe rust
Resistance gene
Genetic analysis
Molecular mapping
