摘要
目的了解副溶血性弧菌食物中毒和临床腹泻株Ⅲ型分泌系统的分布以及耐药特征。方法对食物中毒和临床腹泻分离到的共21株副溶血性弧菌进行毒力基因tdh、trh、T3SS1、T3SS2α、T3SS2β和toxR检测,并用VITEK 2 compact全自动微生物鉴定系统进行了耐药性分析。结果 21株菌株中tdh+/trh-占90.48%(19/21),tdh-/trh+和tdh-/trh-分别占4.76%、4.76%,未检测到tdh+/trh+菌株。T3SS1广泛存在于所有菌株中。T3SS2α存在于tdh+/trh-菌株,T3SS2β存在于tdh-/trh+菌株。1株食物中毒菌株毒力基因携带情况为tdh-/trh-/T3SS2α-/T3SS2β-。21株副溶血性弧菌对阿莫西林、头孢吡肟、抗菌素B、庆大霉素、环丙沙星和复方新诺明敏感,对氨苄西林完全耐药。结论食物中毒和临床腹泻分离到的菌株大多携带tdh基因,T3SS2α与tdh相关,而T3SS2β则存在于trh+菌株。未携带tdh和trh基因的食物中毒分离株表明副溶血性弧菌不仅仅依赖TDH和TRH发挥毒力作用,其致病机制具有多样性和复杂性。
Objective To investigate the distribution of type Ⅲ secretion system and antimicrobial resistance of Vibiro parahemolyticus. Methods The strains were isolated from food poison cases and clinical diarrhea patients.21 strains were examined for the tdh,trh,T3SS1,T3SS2α,T3SS2β and toxR by PCR. The VITEK system was adopted for the detection of antimicrobial resistance. Results 19（ 90. 48%） isolates were tdh＋/ trh-,1（ 4. 76%）isolate from clinical diarrhea was tdh-/ trh＋and 1（ 4. 76%） strain from food poison cases was tdh-/ trh-. T3SS1 existed ubiquitously in the 21 isolates. T3SS2α was shown to be associated with tdh＋/ trh-,and was found in all the19 tdh＋/ trh-strains. T3SS2β was present in tdh-/ trh＋strains. The tdh-/ trh-isolate was negative for T3SS2α and T3SS2β. All the 21 isolates were sensitive to amoxicillin,cefepime,amikacin,gentamicin,ciprofloxacin and trimethoprim,while resistant to ampicillin. Conclusion The isolates from different origins were mostly（ 90. 48%）tdh＋. The tdh was associated with T3SS2α and trh with T3SS2β. The tdh-/ trh-/ T3SS2α-/ T3SS2β-isolate indicated that there are other virulence factors remained to be identified.
出处
《中国微生态学杂志》
CAS
CSCD
2015年第2期159-162,共4页
Chinese Journal of Microecology
