摘要
【目的】研究不同运动强度对红细胞膜Na+、K+-ATP酶活性及红细胞膜脂质成分的影响,探讨运动对红细胞形态和功能影响的机制,进而探讨疲劳发生的可能机制。【方法】武警指挥院校学员,选取3种训练科目,用Polar表记录心率,分别在3种运动负荷前后采集静脉血,采用比色法红细胞膜Na+、K+-ATP酶活性;采用邻苯二甲醛法测定红细胞膜胆固醇含量;采用定磷法测定红细胞膜磷脂含量。【结果】在中、大强度运动范围,Na+、K+-ATP酶活性升高,胆固醇及磷脂含量降低(P<0.05),胆固醇/磷脂比值无明显变化;在极限强度运动范围,Na+、K+-ATP酶活性下降,胆固醇及磷脂含量进一步降低(P<0.01),胆固醇/磷脂比值降低(P<0.05)。【结论】红细胞膜胆固醇/磷脂含量影响红细胞膜Na+、K+-ATP酶活性,表现为双向态势。在中、大强度运动范围呈代偿性影响,可能与运动适应有关。在极限强度运动范围,呈协同下降影响,可能与运动疲劳形成有关。
【Objective】To investigate the effects of exercises with different loads on Na＋-K＋-ATPase activity and lipid component in human erythrocyte membrane and explore the mechanism of fatigue.【Methods】The testing members of armed police forces schools chose three training subjects respectively. Polar Heart Rate Monitor was used to record heart rate. Venous blood was phlebotomized before and after the 3 trainings. Na＋-K＋-ATPase activity was measured by colorimetry. Membrane cholesterin content was determined by phthalic aldehyde method. Membrane phospholipid content was measured by inorganic phosphate method.【Results】After medium and high intensity exercises, Na＋- K＋- ATPase activity remained high, whereas cholesterol and phospholipid contents decreased significantly（P〈0.05） and their ratio appeared no obvious difference. After heavy-load exercises, erythrocyte Na＋-K＋-ATPase activity reduced; content of cholesterol and phospholipid decreased more（P〈0.01） and their ratio also descended（P〈0.05）.【Conclusion】There is a reversible phenomenon of cholesterol and phospholipid content and Na＋-K＋-ATPase activity. It may relate to adaptation in medium and high loads and fatigue in heavy load.
出处
《武警后勤学院学报（医学版）》
CAS
2015年第1期21-23,共3页
Journal of Logistics University of PAP（Medical Sciences）
基金
武警后勤学院院级课题项目(WHM201205)
