摘要
目的探讨姜黄素对顺铂所致小鼠肝损伤的防护效应。方法 30只SD雄性小鼠被随机分为对照组、顺铂组、联合用药组3组。顺铂组给予顺铂腹腔注射;联合用药组先给予姜黄素液灌胃10 d,而后给予顺铂腹腔注射。末次注射24 h后,检测各组小鼠肝/体质量比,血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和肝匀浆中的琥珀酸脱氢酶(SDH)、Na+,K+-ATP酶活性,并观察比较肝组织的形态学变化。结果顺铂组小鼠血清中ALT和AST活性显著增高;肝匀浆中的SDH和Na+,K+-ATP酶活性明显降低,肝细胞呈现气球样变。联合用药组小鼠注射顺铂后上述变化均明显减轻(P<0.01或P<0.05)。结论口服姜黄素对顺铂所致小鼠肝损伤有防护效应。
Objective It is to investigate the protective effect of curcumin on cisplatin-induced liver injury in mice.Methods 30 male SD mice were randomly divided into three groups： control group,cisplatin group and combination of curcumin with cisplatin group.Cisplatin group was given intraperitoneal injection of cisplatin,combination therapy group was first orally administered with curcumin for 10 days and then given intraperitoneal injection of cisplatin.At 24 hours after the last injection,mice were sacrificed and the livers were weighed to calculate the weight ratio of liver/body.Alanine aminotransferase（ALT）,aspartate aminotransferase（AST） activities in serum and the succinate dehydrogenase（SDH）,Na＋.K＋-ATPase activities in liver homogenate were detected by spectrophotometric method,and the morphological changes of liver tissue were observed.Results In cisplatin group,the activities of ALT and AST in serum were significantly increased,while the activities of SDH and Na＋,K＋-ATPase in liver homogenate were also significantly reduced;most of the liver cells showed balloon-like degeneration.In the combination group,the changes above-mentioned were obviously relieved（P0.01 or P0.05）.Conclusion Orally administration with curcumin can protect the liver injury by cisplatin in mice.
出处
《现代中西医结合杂志》
CAS
2010年第34期4401-4403,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
关键词
姜黄素
顺铂
肝损伤
curcumin
cisplatin
liver injury
