摘要
目的 :研究蛙皮素 (BBS)对失血感染模型大鼠肠黏膜屏障和肠道细菌移位的影响。方法 :从颈静脉插管放血制成单纯失血模型 ;从十二指肠插管灌注大肠杆菌液制成单纯感染模型 ;放血加十二指肠灌注大肠杆菌液制成失血感染模型。 80只大鼠随机分为假手术组、单纯失血组、单纯感染组、失血感染组和不同浓度的BBS干预组共 7组 ,BBS干预组分别用 5、2 0和 80 μg/kg剂量给失血感染大鼠皮下注射。观察大鼠肠系膜淋巴结 (ML N)和肝脏组织匀浆体外培养后的细菌量、细菌移位率以及门静脉内毒素水平。结果 :假手术组有16 .7%的组织培养为细菌阳性 ,单纯失血组和单纯感染组各有 5 0 .0 %大鼠组织细菌阳性 ,失血感染组组织细菌培养的阳性率达 95 .8% ,BBS各干预组阳性率则分别为 16 .7%、16 .7%和 72 .7% ,其中 5和 2 0μg/kg干预组与失血感染组相比均有显著性差异 (P均 <0 .0 1)。 5和 2 0μg/kg干预组肝及 ML N组织平均含菌量与失血感染组相比均有显著性差异 (P均 <0 .0 5 )。内毒素水平在 5和 2 0 μg/kg BBS干预组内毒素水平明显低于失血感染组 (P均 <0 .0 1)。结论 :一定剂量的
Objective:To study the effects of bombesin on bacterial translocation in rats after hemorrhage combined with infection.Methods:Eighty Wistar rats were divided randomly into 7 groups.Different doses of bombesin (5,20 and 80 μg/kg) or saline were injected subcutaneously in rats subjected to hemorrhage combined with infection or controls.Bacterial cultures were performed together with the measurement of portal endotoxin level in all animals.Results:The incidence of bacterial translocation was 95 8% in hemorrhage combined with infection group,while it was 16 7% and 16 7% in 5 and 20 μg/kg bombesintreated groups,respectively.The endotoxin levels were significantly lower in 5 and 20 μg/kg bombesintreated groups than that in hemorrhageinfection groups (all P <0 05).Conclusions:These data suggest that treatment with bombesin in certain doses can prevent intestinal bacterial translocation following hemorrhage combined with infection.
出处
《中国危重病急救医学》
CAS
CSCD
2002年第8期496-498,共3页
Chinese Critical Care Medicine
基金
华西医科大学硕士研究生毕业论文基金
