摘要
目的建立糖智宁胶囊的定量分析方法。方法采用HPLC测定糖智宁胶囊中葛根素、盐酸小檗碱、人参皂苷Ro和竹节参皂苷Ⅳa的含量。Kromasil C18色谱柱（4.6 mm×250 mm,5μm）;柱温30℃;流动相为乙腈-0.2%磷酸溶液（梯度洗脱）;流速1 ml·min-1;检测波长203 nm。结果 HPLC中,葛根素、盐酸小檗碱、人参皂苷Ro和竹节参皂苷Ⅳa线性范围分别为0.87∽15.66μg（r=0.999 6）、1.84-40.48μg（r=1.000 0）、0.19-4.114μg（r=0.999 9）、3.24-71.28μg（r=0.999 9）,平均加样回收率分别为98.14%、98.18%、98.69%、97.02%,RSD分别为0.51%、2.16%、1.39%、1.50%。结论该方法简单、专属性强、重复性好、结果准确可靠,可用于糖智宁胶囊的质量评价。
Objective To establish the quantitive research standard of Tangzhining capsule. Methods TLC was employed to identify Puerariae Radix and Panacis majoris Rhizome; Puerarin,berberine hydrochloride,ginsenoside Ro and chikusetsusaponinⅣ a were determined by HPLC. The chromatographic separation was Kromasil C18column（ 4. 6 mm × 250 mm,5 μm）; the column temperature was maintained at 30℃; The mobile phase was acetonitrile-0. 2% phosphoric acid aqueous solution in a gradient elution mode; the flow rate was 1 mL·min-1; and detection wavelength was set at 203 nm. Results TLC spots were clear,well-separated and specific without interference of the negative control. Puerarin,berberine hydrochloride,ginsenoside Ro and chikusetsusaponin Ⅳa had good linearity in the ranges of 0. 87 ∽ 15. 66 μg（ r = 0. 9996）,1. 84 ∽ 40. 48 μg（ r = 1. 0000）,0. 19 ∽4. 114 μg（ r = 0. 9999）,3. 24 ∽ 71. 28 μg（ r = 0. 9999）,respectively. The average recoveries were 98. 14%（ 0. 51%）,98. 18%（ 2.16%）,98.69%（ 1.39%） and 97. 02%（ 1. 50%）,respectively. Conclusion The established method is simple,accurate and reproduceable,it is suitable to be used for evaluation of quality of Tangzhining capsule.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2014年第10期2396-2398,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.81102805/H2804)
陕西省科技计划项目(No.2011K16-04-04)
