摘要
目的研究间充质干细胞(MSCs)移植在肺气肿大鼠氧化应激和肺气肿发展过程中的作用。方法 26只雌性SD大鼠随机分为对照组(A组,n=8),肺气肿组(B组,n=8),肺气肿+MSCs移植组(C组,n=10),将B与C组大鼠在香烟烟雾中暴露14周,制作肺气肿大鼠模型,C组大鼠予MSCs移植。完成4次MSCs移植后的2、4周分别处死C组大鼠各1只,观察MSCs在肺气肿大鼠肺部的定植情况;MSCs移植8周后同时处死各组大鼠,观察大鼠肺组织病理形态,并进行定量分析;用硫代戊巴比妥法测定血清和肺组织匀浆中丙二醛(MDA)的水平,用WST-1法测定各组血清和肺组织匀浆中超氧化物歧化酶(SOD)的水平。结果 MSCs移植后2周及4周,C组大鼠肺组织内均可见标记过的MSCs,随着时间的推移,肺组织内MSCs比例逐渐减少。B组和C组大鼠的肺组织呈现肺气肿样改变,B组和C组大鼠肺组织的平均内衬间隔(MLI)高于A组,平均肺泡数(MAN)明显低于A组,B组大鼠肺组织的MLI高于C组,MAN低于C组(P<0.05)。B组和C组大鼠血清及肺组织中MDA高于A组,SOD低于A组,B组血清及肺组织中MDA高于C组,SOD低于C组(P<0.05)。结论 MSCs移植可能通过降低氧化应激水平对大鼠肺气肿发挥治疗作用。
Objective To test the effect of bone marrow mesenehymal stem cells（MSCs） transplantation on oxidativestress and the development of pulmonary emphysema in rats. Methods SD rats（n=26） were randomly divided into threegroups：normal control group（group A, n=8）,emphysema group（group B, n=8） and emphysema＋MSCs transplantation group（group C, n=10）.Rat models of emphysema was established by exposing rats to cigarette smoking for 14 weeks. Then rats ofgroup C received MSCs transplantation. At the 14 thand 28thdays after 4 course of MSCs transplantations, one rat in group Cwas sacrificed at each time point and their lungs were preserved in frozen sections. Survival of MSCs in the lung tissues wereobserved by fluorescence microscopy. Eight weeks after transplantations, lung sections were stained by hematoxylin and eo-sin（HE） to observe the morphological alterations.Mean linear intercept（MLI） and mean alveolar numbers（MAN） were alsomeasured. Serum and lung malondialdehyde（MDA） levels and superoxide dismutase（SOD） activity were also examined. Results At the 14 thday and 28 thday after transplantations of MSCs, MSCs successfully localized to lung and survived in ratmodels of emphysema. Emphysematous changes of lung tissues were observed in both group B and group C. MLI was higherwhile MAN was lower in group B and C than those in group A（P〈0.05）. MLI and MDA levels in serum and lung were high-er while MAN level and SOD activity were lower in group B than those in group C（P〈0.05）.MDA levels in serum and lungwas higher while SOD activity was lower in group B and C than those in group A（P〈0.05）. Conclusion MSCs transplanta-tions can effectively alleviates pulmonary emphysema in rat models which might through reducing oxidative stress.
出处
《天津医药》
CAS
2015年第2期121-124,I0002,共5页
Tianjin Medical Journal
基金
云南省应用基础研究项目资助(2012FB106)
关键词
肺气肿
间质干细胞
氧化性应激
间质干细胞移植
pulmonary emphysema
mesenchymal stem cells
oxidative stress
mesenchymal stem cell transplantation
