摘要
目的 研究骨髓间充质干细胞（BMSCs）联合青蒿琥酯（Art）对肝纤维化大鼠模型的治疗效果.方法 120只SD大鼠,随机抽取20只作健康对照组,另随机取10只处死后取骨髓作BMSCs培养,剩下的90只采用四氯化碳（CCl4）腹腔注射构建大鼠肝纤维化模型,10周后随机处死10只,取肝组织作病理观察以验证肝纤维化模型制作成功.其余80只按数字表法随机分为模型组、Art治疗组、BMSCs移植组和联合治疗组,每组20只.Masson染色观察肝组织纤维化改变,RT-PCR法检测肝组织α-平滑肌肌动蛋白（α-SMA） mRNA、TGF-β1 mRNA的表达,采用酶消化法检测羟脯氨酸（Hyp）含量,血清TNF-α、TGF-β1检测采用酶免法,血清肝纤维化指标检测采用化学发光法,肝功能指标采用生化法检测.结果 联合治疗组肝功能指标明显优于其他组,联合治疗组白蛋白（ALB）为（29.13 ±3.48） g/L,显著高于Art治疗组[（26.31±2.42） g/L]、BMSCs移植组[（24.35 ±3.12）g/L]和模型组[（20.14 ±2.86）g/L],差异均有统计学意义（q=6.27 ～ 19.01,均P＜0.05）;联合治疗组肝纤维化指标显著低于其他组,联合治疗组Hyp为（1.03 ±0.16） μg/L,显著低于Art治疗组[（1.56±0.19） μg/L]、BMSCs移植组[（1.45±0.24） μg/L]和模型组[（3.57 ±0.91）μg/L],差异均有统计学意义（q =20.47 ～27.70,均P＜0.05）;联合治疗组肝组织TGF-β1 mRNA表达强度明显低于其它组,联合治疗组肝组织电泳条带灰度值占对照条带灰度值百分比为（20.16 ±5.21）,显著低于Art治疗组（58.15±9.16）、BMSCs移植组（46.31±6.82）和模型组（97.08±14.23）,差异均有统计学意义（q=20.35 ～44.93,均P ＜0.05）.结论 BMSCs联合Art在肝纤维化治疗过程中有较好的协同作用.
Objective To study the effects of bone marrow mesenchymal stem cells（BMSCs） transplantation combined with artesunate（Art） on rat model of liver fibrosis.Methods 20 rats from 120 SD rats were selected randomly as normal control group.10 rats from those were executed and extracted marrow to culture BMSCs.The other 90 rats were injected intraperitoneally with CCl4 to make liver fibrosis rat models.10 rats from the 90 rats were selected randomly to ensure the liver fibrosis model was made successfully after 10 weeks.Then,the rest 80 rats were divided randomly into 4 groups：model group,Art treated group,BMSCs transplantation group and Art ＋ BMSCs transplantation group.Fibrous degeneration of the hepatic tissue was detected by Masson stain.The mRNA expression of α-SMA,TGF-β1 in liver were detected by RT-PCR.The hydroxyproline（Hyp） level in liver was detected by enzyme digestion method.Serum TNF-αand TGF-β1 were detected by ELISA.Serum hepatic fibrosis parameters were detected by chemiluminescence and serum liver functional parameters were detected by biochemistry method.Results Liver functional parameters of Art ＋ BMSCs transplantation group were better than those of the other groups.ALB in Art ＋ BMSCs transplantation group was （29.13 ± 3.48） g/L,which was significantly higher than （26.31 ± 2.42） g/L in Art treated group （24.35 ±3.12）g/L in BMSCs transplantation group and （20.14 ±2.86） g/L in model group.There were significant differences among the 4 groups（q =6.27 ～ 19.01,all P 〈 0.05）.Liver fibrosis parameters of Art ＋ BMSCs transplantation group were better than other groups.Hyp in Art ＋ BMSCs transplantation group was （1.03 ±0.16） μg/L,which was significantly lower than （1.56 ± 0.19） μg/L in Art treated group,（1.45 ± 0.24） μg/L in the BMSCs transplantation group and （3.57 ±0.91） μg/L in the model group.There were significant differences among the 4 groups （q =20.47 ～ 27.70,all P 〈 0.05）.The expression of TGF-β1 mRNA of Art ＋
出处
《中国基层医药》
CAS
2014年第13期1936-1939,I0005,共5页
Chinese Journal of Primary Medicine and Pharmacy
基金
南京军区医学科技创新经费资助重点课题（11Z012）
关键词
青蒿琥酯
间质干细胞移植
肝硬化
Artesunate
Mesenchymal stem cell transplantation
Liver cirrhosis
