摘要
目的 评估实时荧光定量聚合酶链反应（FQ-PCR）检测临床标本中耐甲氧西林金黄色葡萄球菌（MRSA）MecA耐药基因的价值。方法 选择该院2013年6~12月125份临床标本,包括血液40份,痰液52份,创面分泌物33份进行FQ-PCR检测和细菌鉴定,分析结果符合率,以检测FQ-PCR的灵敏度及特异性。结果 FQ-PCR的灵敏度为0.159pg/μL,检测经细菌培养鉴定的菌株,特异性达到100%,与临床标本结果符合率为97.6%。结论 FQ-PCR检测MRSA的MecA基因更加方便、快速,且灵敏度和特异性等性能指标均比较理想,与细菌鉴定结果符合率较高,适合临床广泛应用。
Objective To evaluate the reliability of fluorescent quantitative polymerase chain reaction（FQPCR）method for detecting methicillin-resistant Staphylococcus aureus（MRSA）MecA resistant gene.Methods A total of 125 clinical specimens were collected from June 2013 to December 2013,including 40 blood specimens,52 sputum specimens,33 specimens of wound secretions.FQ-PCR method and bacteria identification were used to detect all the specimens,and then analyze the consistent rate of results,sensitivity and specifity.Results The sensitivity of FQ-PCR method was 0.159pg/μL,the specificity of FQ-PCR in detecting bacterial strain cultured and identified was100%,and the consistent rate with the results of clinical samples was 97.6%.Conclusion FQ-PCR method is more convenient,fast in detecting MecA gene of MRSA,and sensitivity,specificity are ideal,the consistent rate with bacterial identification result is high,so could be used widely in clinical applications.
出处
《检验医学与临床》
CAS
2015年第4期436-438,共3页
Laboratory Medicine and Clinic
基金
全军十二五面上课题(CWS11J205)
