摘要
目的:研究育阴软肝方对小鼠化学性急性肝损伤的保护作用。方法:将55只昆明小鼠随机分为正常对照组、模型对照组、育阴软肝方低、中、高剂量组(n=11),连续给药7 d后,采用腹腔分别注射D-半乳糖胺(DGal N)、四氯化碳(CCl4)或硫代乙酰胺(TAA)复制三种肝损伤模型,通过测定血液生化指标、肝组织抗氧化酶及氧化产物的水平,研究育阴软肝方对实验性肝损伤的保护作用。结果:育阴软肝方能降低D-Gal N、CCl4或TAA诱发急性肝损伤小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)的水平,提高急性肝损伤小鼠肝组织超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性,降低丙二醛(MDA)的含量。其降酶保肝、抗氧化作用显示出一定的量效关系。结论:育阴软肝方对实验性急性肝损伤具有一定的保护作用。
Objective： To investigate the protective effects of Yuyin Ruangan Decoction（ YRD,traditional Chinese medicine） on experimental hepatic injury in mice. Methods： The mice were randomly divided into control group,model group and YRD low,middle and high dose group（ n = 11）. By ip injection of D-Gal N,CCl4 or thioacetamide（ TAA）,three models of hepatic injury mice were established to investigate the effects of YRD through detecting the indexes of liver function in serum and,the content of antioxidant system in the hepatic tissue. Results： YRD could decrease the content of alanine aminotransferase（ ALT） and aspartate aminotransferase（ AST） in serum and that of malonaldehyde（ MDA） in the hepatic tissue,upregulate the activity of superoxide dismutase（ SOD） and glutathione peroxidase（ GSH-Px） in the hepatic tissue. Furthermore,the above effects were dosedependent in a certain degree. Conclusion： YRD has some protection effects on the model of experimental hepatic injury in mouse.
出处
《中国应用生理学杂志》
CAS
CSCD
2015年第1期76-79,共4页
Chinese Journal of Applied Physiology
关键词
育阴软肝方
肝损伤
抗氧化系统
小鼠
Yuyin Ruangan Decoction（YRD）
hepatic injury
antioxidant system
rats
