摘要
目的:建立2型糖尿病合并抑郁症大鼠模型并进行评价,为进一步研究该病提供动物模型。方法:将24只Wistar大鼠随机分为3组(n=8):正常组(N组)、2型糖尿病组(T组)、2型糖尿病合并抑郁症组(T+D组)。采用高脂饲料喂养加腹腔注射小剂量链脲佐菌素(STZ)的方法制备2型糖尿病模型,在其基础上再用21 d慢性束缚的方法建立2型糖尿病合并抑郁症大鼠模型。21 d慢性束缚后做口服葡萄糖耐受试验(OGTT)和胰岛素耐受试验(ITT);利用行为学设备对大鼠旷场实验进行分析,了解大鼠的抑郁程度;取材后用ELISA法检测大鼠血清5-羟色胺(5-HT)和多巴胺(DA),验证评价模型是否成功。结果:在ITT和OGTT中,T组和T+D组的血糖在180 min后没有恢复到正常水平;旷场行为学分析表明T+D组大鼠5 min内最大移动距离,中央区滞留时间和中央区滞留次数降低;T+D组血清中5-HT和DA含量降低。结论:本实验利用高脂饲料喂养加腹腔注射小剂量STZ及21 d慢性束缚的方法,成功复制2型糖尿病合并抑郁症大鼠模型,适用于后续研究。
Objective： To establish and evaluate a rat model of type 2 diabetes mellitus（ T2DM） associated with depression for further elaborating the disease. Methods： Twenty-four Wistar rats were randomly divided into three groups： normal group（ group N）,T2 DM group（ group T） and T2 DM with depression group（ group T ＋ D）,with 8 rats in each group. The T2 DM rat model was induced by high fat diet and low dose of Streptozotocin（ STZ） injection,and in addition,the T2 DM rats were made restraint stress for 21 days.After the model was established,the insulin tolerance test（ ITT） and oral glucose tolerance test（ OGTT） were performed. Then the rat depression level was analyzed by open field test,and the concentration of 5-hydroxytryptamine（ 5-HT） and dopamine（ DA） was determined by ELISA to confirm the model identity. Results： The blood glucose level in group T and group T ＋ D didn＇t return to the normal level at 180 minutes in the ITT and OGTT test; Compared with the group N,the max movement distance,retaining time in the central zone and the retaining frequency within 5 minutes in the group T ＋ D decreased; 5-HT and DA level in the serum of rats in group T ＋ D was reduced. Conclusion： A rat model of type 2 diabetes mellitus associated with depression has been successfully established by high fat diet and injection of low dose streptozotocin in combination with restraint stress for 21 days. This rat model is useful for further relevant studies.
出处
《中国应用生理学杂志》
CAS
CSCD
2015年第1期23-26,I0003,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金项目(81072756)
国家自然科学基金资助项目(81473597)
北京中医药大学创新团队项目资助(2011CXTD-07)
