摘要
目的:探讨银杏叶提取物(EGB)对Ⅰ型糖尿病大鼠海马神经元神经生长因子(NGF)和神经营养因子-3(NT-3)表达的影响。方法:30只雄性SD大鼠随机平分为3组(n=10):CON组,DM组,EGB组。应用链脲佐菌素腹腔注射诱导构建糖尿病大鼠模型,EGB组腹腔注射EGB注射液,其余2组给予同容积的生理盐水。动态观察大鼠行为、体重及血糖水平的变化。于12周末用Morris水迷宫检测大鼠学习记忆能力,常规HE染色观察海马组织形态学改变,用Western bot和RT-PCR法检测大鼠海马组织中NGF、NT-3的表达。结果:与糖尿病组相比,EGB组大鼠行为、体重(P<0.05)和血糖(P<0.05)明显改善;Morris水迷宫潜伏期明显缩短(P<0.05)、搜索策略能力明显提高(P<0.01);HE染色显微镜观察海马组织病理改变减轻;Western bot和RT-PCR分析表明EGB组大鼠海马组织中NGF、NT-3表达均明显增高(P<0.05)。结论:EGB改善糖尿病大鼠的认知功能,其机制可能与EGB增加海马神经元NGF、NT-3的表达,减少神经元凋亡有关。
Objective： To investigate the effect of extract of Ginkgo Biloba（EGB） on nerve growth factor（NGF） and Neurotrophin-3（NT-3） expression of hippocampus neurons in streptozotocin-induced type I diabtic rats.Methods： Thirty male SD rats were divided into three groups（n=10）： the control group,diabetic group and EGB-treated group.Strepozotocin were injected intraperitoneally in the later two groups to induce diabetes.EGB-treated group was injected intraperitoneally with EGB,and the same volume of normal saline was injected to the other groups.Concentration of blood glucose and body weight and behaviour were dynamicly monitored.At the end of the 12th week,morphological changes of the hippocampus neurons were observed under microscopy by HE stain.The expression of NGF and NT-3 were assayed by Western blot and RT-PCR respectively.Results： Compared with diabetic group,the behaviour and body weight（P〈0.05） and the concentration of blood glucose（P〈0.05） were significantly improved and the escape latency of Morris water maze test（P〈0.05） was significantly shortened,while the platform searching score was significantly increaseed（P〈0.01） in EGB treated group;The pathological changes of hippocampus neurons were significantly attenuate by EGB treated;The expression of NGF and NT-3 in hippocampus neurons were significantly increased which assayed by Western blotting and RT-PCR respectively（P〈0.05） in EGB treated group.Conclusion： EGB may improve the learning and memory ability of biabetic rats the mechanism may be attributed to its improvement of the expression of NGF and NT-3 and reducing apoptosis in hippocampus neurons.
出处
《中国应用生理学杂志》
CAS
CSCD
2012年第5期467-471,491,共5页
Chinese Journal of Applied Physiology
基金
浙江省温州市科技计划中外合作项目(H20090008)
关键词
银杏叶提取物
糖尿病脑病
神经营养因子
海马
extract of Ginkgo Biloba
diabetic encephalopathy
neurotrophic factor
hippocampus
