摘要
背景:异种冻干骨具有很好的骨引导性及低抗原性,但骨诱导性相对较差;自体富血小板纤维蛋白具有很好的诱导成骨能力。目的:观察富血小板纤维蛋白/异种冻干骨复合物在修复种植体周骨缺损及骨整合过程中的作用。方法:取新西兰大白兔12只,于双侧下颌无牙区造极限骨缺损模型。在两侧缺损区的近中、远中端骨壁各植入1枚钛螺纹钉,使种植体临近缺损侧无骨支持,右侧缺损区填压入自体富血小板纤维蛋白/异种冻干骨复合物,作为实验组;左侧缺损区填入异种冻干骨,作为对照组。植入4,8,12周后取完整下颌骨标本进行大体形态观察、植体扭矩测试及组织学观察。结果与结论:植入4周时,实验组可见新生血管,成骨细胞数量多,呈单层紧密排列在新生骨小梁的表面;对照组可见炎性细胞浸润,纤维组织增多,新生血管稀疏,成骨细胞及新生骨数量较实验组少。植入8周时,实验组见大量新生血管,骨小梁密集,呈网状连接,部分已逐渐融合成岛状;对照组新生血管较实验组少,骨小梁纤细,散乱。植入12周时,实验组新生骨组织成熟,骨小梁钙化程度高,可见形成板状新骨;对照组骨小梁融合成片,趋向成熟,但成熟度不及实验组。实验组不同时间点种植体-骨结合强度高于对照组(P<0.05)。表明富血小板纤维蛋白/异种冻干骨复合物具有很好的骨修复与骨整合能力。
BACKGROUND： Freeze-dried bone xenograft has good osteoconductive ability and low antigenicity, but exhibits relatively poor osteoinductive ability. However, autologous platelet rich fibrin possesses good osteoinductive ability.
OBJECTIVE： To observe the effect of freeze-dried bone xenograft/platelet rich fibrin compound on bone defect repairing and osseointegration around the implant.
METHODS： Twelve normal New Zealand white rabbits were used to prepare the bone defect models at the edentulous area of the bilateral mandible. One titanium screw was implanted in the mesial and distal bone wall of bilateral bone defects respectively. The defects in the right side as experimental group were filled with freeze-dried bone xenograft/platelet rich fibrin compound, while the left defects as control group were inserted only by the freeze-dried bone xenograft. The intact mandible specimens were harvested at 4, 8, 12 weeks after implantation separately, and tested by gross observation, torque test and histological observation.
RESULTS AND CONCLUSION： At 4 weeks after implantation, the experimental group had new vessel formation and a large amount of osteoblasts that were arranged tightly within the monolayer on the surface of newborn bone trabecula; the control group was found to have inflammatory cell infiltration, an increase in fibrous tissue and sparse new vessels, but the number of osteoblasts and new bones was less than that of the experimental group. At 8 weeks after implantation, a great amount of new vessels were seen in the experimental group, and dense bone trabeculae exhibited a mesh-like interconnection, part of which were gradually fused in an island-like shape; in the control group, the number of new vessels was less than that of the experimental group, and the bone trabeculae were slim and scattered. At 12 weeks after implantation, the newborn bone tissue was mature and highly calcified bone trabeculae were seen in the experimental group with formation of lamellar bone; in the control group, the b
出处
《中国组织工程研究》
CAS
CSCD
2014年第52期8376-8381,共6页
Chinese Journal of Tissue Engineering Research
