摘要
背景:通过药物治疗虽然能在一定程度上减轻和延缓阿尔茨海默病进展,但是效果不明显,因此采用细胞替代治疗是目前治疗该疾病的一种新的尝试和探索。目的:观察骨髓间充质干细胞移植对阿尔茨海默病大鼠记忆能力改善的影响。方法:注射β淀粉样蛋白1-40制备阿尔茨海默病动物模型并于双侧海马区移植骨髓间充质干细胞。治疗4周后采用Morris水迷宫检测大鼠空间认知和记忆能力变化,采用Brd U和NF,GFAP的免疫荧光双标技术观察移植的细胞是否存活并分化,免疫印迹和免疫组化技术检测大脑皮质及海马区β淀粉样蛋白表达。结果与结论:与单纯造模组比较,移植骨髓间充质干细胞治疗后大鼠逃避潜伏期明显缩短(P<0.01),跨越平台次数明显均多(P<0.05)。免疫荧光双标显示,移植细胞在大鼠海马周围分化为NF或GFAP阳性的细胞。免疫组化和免疫印迹分析显示,经骨髓间充质干细胞移植治疗后,其脑内β淀粉样蛋白表达明显下降(P<0.05)。结果表明骨髓间充质干细胞移植后能在阿尔茨海默病大鼠脑内局部存活并分化,并且能够提高阿尔茨海默病大鼠学习记忆能力。
BACKGROUND： Although medications can reduce and delay the progression of Alzheimer＇s disease to a certain extent, but the effect is not obvious. Therefore, cell replacement therapy is a new attempt and exploration for the treatment of Alzheimer＇s disease. OBJECTIVE： To study the improvement in learning and memory abilities of rats with AIzheimer＇s disease after transplantation of bone marrow mesenchymal stern cells. METHODS： Alzheirner＇s disease model was induced by injecting beta-amyloid 1-40 protein into the bilateral hippocarnpi. The bone marrow mesenchymal stem cells were then implanted into the hippocampus. After 4 weeks, the spatial cognition and memory ability of rats were evaluated with Morris water maze. BrdU and NF, GFAP irnrnunofiuorescence double-staining was adopted to test the differentiation and survival of implanted bone marrow mesenchyrnal stem cells. Western blot and imrnunohistochemistry methods were used to detect the expression of beta-amyloid protein in the cortex and hippocampus. RESULTS AND CONCLUSION： Compared with the model group, the escape latency of hidden platform was shortened significantly in the stem cell transplantation group （P 〈 0.01）, while the number of crossing the platform was increased significantly （P 〈 0.05）. The BrdU/NSE and BrdU/GFAP double staining positive cells could be found around the bilateral hippocampi. Western blot result showed the beta-amyloid protein expression decreased obviously after transplantation of bone marrow rnesenchyrnal stem cells （P 〈 0.05）. These findings indicate that transplanted bone marrow mesenchymal stern cells can survive and differentiate in the brain of rats, which can improve the learning and memory abilities of Alzheirner＇s disease rats.
出处
《中国组织工程研究》
CAS
CSCD
2014年第50期8130-8134,共5页
Chinese Journal of Tissue Engineering Research
