摘要
为了研究不同轮回选择方法对玉米群体遗传多样性的影响,本研究利用SSR分子标记分析了基础群体P4C0及其经过不同轮回选择方法改良群体的遗传多样性。研究结果表明,P4C0经过5轮控制双亲混合选择(MS)后,在群体改良的低代,群体遗传多样性得到了较好的保持,而多代的改良导致群体遗传多样性下降。P4C0经过1轮半同胞-S2∶3(HS-S2∶3)选择后,遗传多样性比P4C0有较大幅度的下降。P4HSC1经过1轮开放改良后,遗传多样性有较大幅度的增加。P4HSC1经过3轮MS改良后,群体遗传多样性呈增大的趋势,但每轮增加的幅度均较小;分子方差分析结果表明,不同轮回选择方法改良后,群体间的遗传变异仍远远小于群体内的遗传变异;主坐标分析结果表明,随着改良轮次的增加,群体内个体的分布发生了定向变化,且不同选择方法改良后,群体内个体偏移的方向和程度不一致。研究结果为这些轮回选择方法的利用和完善提供了依据。
In order to study the effect of different recurrent selection methods on population genetic diversity,SSR molecular markers were used to analyze the genetic diversity of a narrow base maize population P4C0 and its improved population obtained by different recurrent selection methods. The results showed that population genetic diversity were kept well at a lower generation of improved population after 5 cycles of biparental mass selection method on base population P4C0. However,it declined at a higher generation of improved population. Compared with P4C0,the genetic diversity of improved population with one cycle of Half Sib-S2∶ 3 selection on P4C0 decreased. For population P4HSC1,the genetic diversity increased dramatically after one cycle of open population improvement,moreover,it would most likely continue at a modest pace after three cycles of MS improvement. The AMOVA（ analysis of molecular variance）results showed that the genetic diversity within population was much higher than those among populations with the advantage of different recurrent selection. In addition,the principal coordinates analysis showed that distribution of individual were directionally variational with the increasing of impovement selection,it also varied with a different individual offset direction and degree after different recurrent selection methods. The results provided a basic for application and improvement of these methods.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第1期21-28,共8页
Journal of Nuclear Agricultural Sciences
基金
国家玉米产业技术体系(CARS-02-07)
“十二五”农村领域国家科技计划课题“强优势玉米杂交种的创制与应用”(2011AA10A103-2)
四川省教育厅资助科研项目(13ZA0250)
关键词
玉米
窄基群体
轮回选择
遗传多样性
SSR
maize
narrow base population
recurrent selection
genetic diversity
SSR
