摘要
目的建立冬凌草甲素纳米粒中主药含量及包封率的测定方法。方法采用高效液相色谱法测定药物含量，DiamonsilC18色谱柱（4．6mm×250mm，5μm）；流动相为甲醇一水（60：40）；流速1．0mL·min^-1；检测波长240nm；柱温30℃；采用葡聚糖凝胶法分离冬凌草甲素纳米粒游离药物以测定包封率。结果冬凌草甲素在2．0～200μg·mL。线性良好（y=0．9999），低、中、高浓度的平均回收率分别102．5％（RSD1．56％）；97．8％（RSD1．37％）；98．3％（RSD0．22％），所测纳米粒的平均包封率为21．8％。结论该方法准确可靠，简单快速，重复性好，可用于冬凌草甲素纳米粒药物含量及包封率的测定。
Objective To establish a method to determine the content and entrapment efficiency of oridonin capsulated in nanoparticles. Method The HPLC analysis was carried out on a Diamonsil C18(4. 6mm×250mm,5 μm) column with mobile phase consisted of methanol-H2O(60:40) at a flowrate of 1. 0 m L·min-1. The detection wave length was at 240 nm and the column temperature was 30℃. The free drug was isolated by Gel-filtration for the entrapment efficiency determination. Result The1 inear range of oridonin was 2.0 ~200μg·m L-1(γ=0. 9999). The average recovery was 102.5%(RSD 1.56%); 97.8%(RSD 1.37%); 98.3%(RSD 0.22%)respectively at low, middle and high concentration;97.8%(RSD 1.37%);98.3%(RSD 0.22%), respectively.The average entrapment efficiency was 21. 8%. Conclusion This method is simple,precise and replicable and it can be used to determine the content of total oridonin in nanoparticles and entrapment efficiency.
出处
《佛山科学技术学院学报（自然科学版）》
CAS
2015年第1期1-4,共4页
Journal of Foshan University(Natural Science Edition)
基金
国家自然科学基金项目(81403111)
广东省自然科学基金项目(S2011040003133)
关键词
冬凌草甲素
纳米粒
含量测定
包封率
oridonin
nanoparticles
content determination
entrapment efficiency
