摘要
目的：研究肽酰基精氨酸脱亚胺酶（PADI-4）在类风湿关节炎（RA）中的诊断意义。方法用酶联免疫吸附试验（ELISA）双抗体夹心法检测58例 RA 患者，40例其他风湿性疾病和30名健康志愿者血清中的 PADI-4、抗核周因子（APF）、抗环瓜氨酸肽（CCP）抗体和抗角蛋白抗体（AKA）的含量，应用 ROC曲线、方差分析和相关性分析对各项指标进行评价。结果 RA患者血清中PADI-4浓度（2.653±2.719 U／L）显著高于其他风湿性疾病组（0.872±0.292 U／L）和健康组（0.793±0.243 U／L），差异有统计学意义（t＝22.732，35.371，P 均＜0.01）；采用受试者工作曲线（ROC），PADI-4诊断 RA的最佳临界值为1.284 U／L，此时的灵敏度为62.1％，特异度为91.4％；PADI-4阳性率（62.1％）与 APF（50％）， AKA （56.9％）之间差异无统计学意义（χ2＝0.322，P＝0.570；χ2＝1.715，P＝0.190），与抗CCP抗体（81.1％）之间差异有统计学意义（χ2＝4.161，P＝0.041）；PADI-4水平与 APF，AKA 之间呈正相关（r＝0.652，0.01，0.666，P＜0.01），与抗CCP抗体之间未显示出相关性（r＝0.122，P＝0.357）。结论 PADI-4在部分 RA患者血清中显著升高，PADI-4对 RA具有良好的灵敏度和特异度，可能成为独立于抗CCP抗体之外的 RA的一种新的诊断标志物。
Objective To investigate the diagnostic value of peptidylarginine deaminase-4 （PADI-4）detected in patients with rheumatoid arthritis （RA）.Methods The levels of PADI-4,anti-CCP antibodies,AKA and APF were detected by ELISA in serum samples from 58 patients with RA,40 other rheumatic disease and 30 healthy individuals.The indicators were evaluate by application of ROC curve analysis,analysis of variance and correlation.Results PADI-4 serum level（2.653±2.719 U/L） of patients with RA was significantly higher than other rheumatic diseases group （0.872 ± 0.292 U/L）and the healthy group （0.793±0.243 U/L）,the difference was statistically significant （t=22.732,35.371,P〈0.01）.Receiver operator curve analysis （ROC）showed an opitium cut off level for PADI-4 at 1.284 U/L,the sensitivity of PADI-4 was 62.1%.The specificity was 91.4% in RA.There was no significant difference between the positive rate of PADI-4 （62.1%）and APF （50%）,AKA （56.9%）（χ2=0.322,P=0.570;χ2=1.715,P=0.190）,and there was asignificant difference between PADI-4 and anti CCP antibody （χ2=4.161,P=0.041）;a positive correlation between PADI-4 and APF,AKA （r=0.652,0.666, P〈0.01）,PADI-4 and between anti CCP antibody showed no correlation （r=0.122,P=0.357）.Conclusion PADI-4 was significantly increased in serum of RA patients in part,PADI-4 has good sensitivity and specificity of RA,a new diagnostic markers might become independent of antibody against CCP in RA.
出处
《现代检验医学杂志》
CAS
2014年第6期81-83,共3页
Journal of Modern Laboratory Medicine
基金
安徽宿州市科技局科研基金资助项目（项目编号：20122017）.
