摘要
以东北地区早春植物早花忍冬的幼嫩茎段为外植体,MS培养基为基本培养基,添加不同的植物生长调节物质,进行了离体快速繁殖技术研究。结果表明:采用2.0%NaClO处理20min的灭菌效果好;诱导早花忍冬侧芽分化的适宜培养基为MS+1.0mg/L 6-BA+0.05mg/L NAA+3.0%蔗糖+0.7%琼脂;增殖培养基为MS+2.0mg/L 6-BA+0.05mg/L NAA+3.0%蔗糖+0.7%琼脂,30d的增殖系数为8;最佳的生根培养基为1/2MS+0.3mg/L IBA+0.3mg/L NAA+1.5%蔗糖+0.7%琼脂,生根率达100%。
Taking tender stem section of Lonicera praeflorens which flowers in early spring in the Northeast as material,MS medium was selected as the basic medium supplemented with different plant growth regulators,the efficient process for the regeneration was studied.The results showed that the surface sterilization in 2.0% sodium hypochlorite for20 minutes was the best.The optimum medium for the differentiation of lateral buds was MS＋1.0 mg/L 6-BA＋0.05mg/L NAA＋3.0%sucrose＋0.7%agar;the medium MS＋2.0mg/L 6-BA＋0.05mg/L NAA＋3.0%sucrose＋0.7%agar was good for multiplication and the proliferation coefficient was 8.The optimum medium for inducing root was1/2MS＋0.3mg/L IBA＋0.3mg/L NAA＋1.5%sucrose＋0.7%agar,and the rooting rate was 100%.
出处
《北方园艺》
CAS
北大核心
2015年第4期95-98,共4页
Northern Horticulture
基金
吉林省教育厅"十二五"科技资助项目(20140172)
关键词
早花忍冬
组织培养
早春植物
Lonicera praeflorens Batalin
tissue culture
early spring plant
