摘要
目的：石榴酸（C18：3）在结构上为顺9，反11，顺13-十八碳三烯酸，为长链烃类，在200-400nm没有吸收，无法直接用紫外检测器进行检测，本实验采用柱前衍生化方法，使石榴酸带上一个强发色团，使其在200-400nm有最大吸收，建立石榴籽油中指标性成分石榴酸的柱前衍生化HPLC分析方法。方法：以ω-溴代苯乙酮作为衍生化试剂，以三乙醇眩为相转移催化剂，对石榴籽油进行柱前衍生化，色谱条件采用Diamonsi C18色谱柱（4.6mm×250mm，5μm），检测波长272nm，以甲醇-乙腈-水（68.5：20：11.5）为流动相，柱温30℃，流速1.0mL·min^-1。结果：石榴酸在0.0266-0.1330g·L^-1（r=0.999）线件关系良好，平均加样回收率为98.7％，RSD1.8％。结论：该法稳定性、重复性好，定量准确，可作为石榴籽油中“榴酸的定量方法，用于评价和控制石榴籽油的质量。
Objective： As the long chain hydrocarbons, punicic acid （C18：3） on the structure of 9 （Z） , 11 （E）, 13 （Z） -octadecatrienoic acid, which was in 200-400 nm absorption-free and can＇t be measured directly by UV detector. This experiment adopted column derivatization methods, made the punicic acid with a strong ehromophore in maximum absorption in 200-400nm. The determination method of punicicacid in pomegranate seed oil by HPLC was established in this thesis. Method： The punicic acid were derivatized with ω-bromoacetophenone as derivative reagent, triethanolamine as catalyst, the Diamonsi Clseolumn （4.6 mm×250 mm, 5μm） was used. The detection wavelength was set at 272 nm, column temperature 30℃, with methanol- acetonitrile-water （68.5： 20：11.5） aseluent, the flow rate was 1.0 mL·min^-1 Result ： The standard curves of punicic acid is linear within in the range of 0.0266-0.1330g·L^-1 and the coefficient is 0.999. The average recovery of punieie acid is 98.7% , RSD is 1.8%. Conclusion： The method is reproducible and accurate, which can be used as the determination method of punicic acid inpomegranate seed oil, and used for the quality control of pomegranate seed oil.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第4期68-70,共3页
Chinese Journal of Experimental Traditional Medical Formulae
