摘要
目的研究石榴籽油与乳腺癌细胞增殖、凋亡行为之间的关系。方法使用气相色谱法检测油脂脂肪酸组成成分;使用乳腺癌MCF-7、MDA-MB-231两种细胞系进行干预,MTT法观察细胞增殖活性,流式细胞术检测细胞凋亡情况,Western印迹法检测细胞内相关增殖及凋亡蛋白的表达。结果石榴籽油中主要的脂肪酸为石榴酸(74.41%);石榴籽油可抑制两种乳腺癌细胞系的增殖,显著导致其细胞凋亡,与对照组相比,差异具有显著性意义;石榴籽油可显著下调细胞中Cox-2、Bcl-2的表达水平,上调Bax、胱天蛋白酶(caspase)-3(酶解)的表达水平,上调MCF-7中P53的表达水平或下调在MDA-MB-231中的表达水平。结论石榴籽油中的石榴酸可能是抑制乳腺癌细胞恶性生物学行为的功能性物质,石榴籽油抑制乳腺癌细胞增殖活性,促进其凋亡作用可能是通过调节细胞中Cox-2、Bcl-2、Bax、胱天蛋白酶-3(酶解)以及P53的表达来实现的。
Objective To study the effect of Punica granatum（ pomegranate） seed oil（ PSO） on proliferation and apoptosis behaviors of breast cancer cells. Methods Fatty acid composition was detected by gas chromatography,breast cancer cells,MCF-7 and MDA-MB-231 were treated with PSO,cell proliferation was observed by MMT,cell apoptosis was analyzed by flow cytometry,and expression levels of proliferation and apoptosis-related proteins were detected by Western blot. Results Punicic acid（ PA） was the major fatty acid in PSO（ 74. 41%）. PSO could inhibit the proliferation while inducing apoptosis in both cell lines in a dose-and time-dependent manner,significantly decrease the expression level of Cox-2 and Bcl-2,increase the expression level of Bax and caspase-3（ cleaved）,remarkably upregulate the expression of P53 in MCF-7,and downregulate p53 expression in MDA-MB-231. Conclusion PA may be one of the functional ingredients of PSO which can inhibit proliferation and induce apoptosis in breast cancer cells. These effects are probably mediated by regulating the expression of Cox-2,Bcl-2,Bax,caspase-3（ cleaved） and p53.
出处
《军事医学》
CAS
CSCD
北大核心
2015年第6期438-442,共5页
Military Medical Sciences
基金
国家自然科学基金资助项目(81102119)
