摘要
采用水提醇沉法提取八角叶多糖,苯酚-硫酸法测定了八角叶的多糖含量。利用正交设计方法优化得到最佳提取工艺条件为:提取温度75℃、料液比1∶50、提取时间3h、提取次数2次,所得八角叶粗多糖产率达12.87%,粗多糖中多糖总含量为28.22%。
The water extraction and alcohol precipitation were selected to extract polysaccharides from illicium verum leaves and the phenol-sulfuric acid method was used to determine the polysaccharide content. The optimal extraction process was selected with the orthogonal test design.The optimal extraction process was extraction temperature 75℃ , with 2h, with 1:50 (g /mL) of illicium verum leaves weight to distilled water and extraction for 2 times. The optimum extraction rate o f polysaccharide from illicium verum leaves was 12.87% and the total content of crude polysaccharides was 28.22%.
出处
《化工技术与开发》
CAS
2015年第1期5-7,共3页
Technology & Development of Chemical Industry
基金
广西自然科学基金资助项目(2013GXNSFAA019033)
广西教育厅科研重点项目(2013ZD038)
广西研究生教育创新计划项目(YCSZ2013087)
关键词
八角叶
多糖
最佳提取工艺
illicium verum leaves
polysaccharide
optimal extraction process