摘要
目的 探讨液氮冻存时间对脐血造血干细胞质量和临床移植效果的影响.方法 对上海脐血库已经应用于临床移植的605份脐血进行研究,脐血平均冻存时间为820（88～ 2651）d,测定脐血复苏之后的有核细胞、CD34＋细胞数量、细胞回收率、细胞活力,粒-单核细胞集落形成单位（CFU-GM）,结合脐血冻存时间、临床移植效果进行统计分析.结果 复苏后细胞回收率、细胞活力与冻存时间无显著相关性,CFU-GM平均（109.6～ 105.7）个/1×10^5,随冻存时间延长逐渐降低（P=0.011）.临床移植患者的造血重建时间、植入失败率、急性移植物抗宿主病、总体生存率与脐血冻存时间长短无显著相关性.结论 深低温冻存时间长短对脐血复苏后细胞回收率、细胞活力、临床移植效果无显著影响,冻存时间较长的脐血复苏后CFU-GM在正常标准范围内略有下降,保存3～7年的脐血仍能维持正常细胞活性和功能、达到良好的临床移植效果.
Objective To investigate the impact of cryopreservation duration of umbilical cord blood （UCB） on quality of hematopoietic stem cell and outcome of clinical transplantation.Methods 605 units of UCB which had been used in clinical transplantation were previously cryopreserved for 820 （88-2651） days in average.UCB was detected for total nucleated cell count,CD34＋ cells count,cell recovery rate,cell viability and CFU-GM after thawing.Results No statistical correlation was found between cryopreservation duration and cell recovery rate,cell viability.CFU-GM decreased along with the extension of cryopreservation duration （P=0.011）,ranging between 109.6 and 105.7/1×10^5.There was no significant difference on hematopoietic reconstitution time,graft failure,acute GVHD and overall survival among groups with different cryopreservation duration.Conclusion Cryopreservation duration has no significant effect on cell recovery rate,cell viability and clinical transplantation outcome.Extension of cryopreservation duration may reduce CFU-GM of stem cells with fluctaion still in normal range.UCB could maintain cell viability and function to achieve satisfactory clinical transplantation outcome even when thawed after 3 to 7 years＇ cryopreservation.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2015年第1期1-3,共3页
Chinese Journal of Hematology
基金
上海市科技成果转化与产业化项目（12431902202）
上海市科技小巨人（培育）企业项目（13HX11810000）
关键词
胎血
深低温保存
造血干细胞
细胞质量
移植预后
Umbilical cord blood
Cryopreservation duration
Hematopoietic stem cell
Cell quality
Outcome
