摘要
目的:探讨Musashi 1在结肠癌中的表达与肿瘤微血管密度(MVD)及预后的关系。方法:应用实时荧光定量PCR的方法检测36对结肠癌组织及其配对的正常结肠组织中Musashi 1 mRNA的表达情况。通过免疫组化法对96例结肠癌组织中Musashi 1表达情况和微血管密度(MVD)进行检测。结合随访术后生存情况,统计分析Musashi 1表达与结肠癌病理参数、预后及MVD之间的相关性。结果:实时荧光定量PCR法结果显示,在结肠癌组织中,Musashi 1 mRNA的相对表达水平显著高于正常结肠组织(P<0.05)。免疫组化结果显示,96例结肠癌组织中,有61例(63.5%)高表达Musashi 1。Musashi1的表达与患者性别、肿瘤大小、分化程度无相关性(P>0.05),而与肿瘤的浸润深度、Dukes分期、淋巴转移、TNM分期密切相关(P<0.05)。Kaplan-Meier生存分析显示,Musashi 1高表达患者的5年生存率显著低于Musashi 1低表达者(23.0%vs60.0%;P<0.01),且Musashi 1的表达与结肠癌血管密度相关(P<0.01)。结论:结肠癌中Musashi 1的表达较正常癌旁组织高,具有统计学意义。结肠癌中Musashi 1的表达与肿瘤浸润深度、Dukes分期、淋巴转移、TNM分期和血管密度密切相关。Musashi 1蛋白可通过促进瘤内血管增生,从而促进结肠癌的生长和侵袭转移,进一步影响结肠癌患者的预后。
Objective： To investigate the Musashi 1 expression,and its correlation with clinical variables,such as clinical pathologic factors,angiogenesis and prognosis in colon cancer. Methods： Musashi 1 mRNA level was determined by quantitative real-time PCR（ qRT-PCR） in 36 pairs of matched colon cancer tissues and adjacent non-tumorous tissues. The expression of Musashi 1 and microvascular density（ MVD） were tested by immunohistochemical in 96 cases of colon cancer. Combined with postoperative follow-up survival situation,the correlation between the expression of Musashi 1,pathological parameters,prognosis and MVD were statistical analylised. Results：Musashi 1 mRNA level of colon cancer tissues determined by PCR was significantly higher than adjacent non-tumorous tissues（ P〈 0. 05）. Positive Musashi 1 immunoreactivity was seen in 63. 5% of colon cancer specimens,which was correlated with the depth of invasion,Duckes stage,lymph node metastasis,and TNM stage（ P〈 0. 05）. Compared with Musashi 1 negative patients,Musashi 1 positive patients had significantly shorter survival time（ 23. 0% vs 60. 0%,P〈 0. 01）. High intratumor blood microvessel density patients had significantly shorter survival times than low intratumor blood microvessel density patients（ P〈 0. 05）. Intratumor blood microvessel density correlated with Musashi 1 expression（ P〈 0. 01）. Conclusion： Musashi 1 mRNA level of colon cancer tissues was higher than adjacent non-tumorous tissues,the difference has statistically significant. We conclude that Musashi 1 expression correlates with the depth of invasion,Dukes stage,lymph node metastasis,and TNM stage and microvascular density（ MVD） in colon cancer. We propose that synthesized Musashi 1 increases intratumor angiogenesis,thus promotes tumor progression. Musashi 1 expression may be a good biomarker for poor prognosis in colon cancer.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2015年第1期97-102,共6页
Chinese Journal of Immunology
基金
浙江省公益技术应用研究项目(No.2013C37066)
