摘要
目的:探讨微小RNA-100(microRNA-100,miRNA-100)在胃癌患者组织中的表达及其与临床病理特征之间的关系。方法:提取50例胃癌及其癌旁非肿瘤组织标本的总RNA,采用Taq Man探针实时荧光定量PCR方法检测miRNA-100的表达量;并分析其与肿瘤分化程度、淋巴结转移等临床病理学特征的关系。结果:胃癌及配对癌旁非肿瘤组织中miRNA-100的相对表达量分别为1.31±0.69和0.74±0.36,差异有统计学意义(P<0.01)。胃癌组织中miRNA-100的表达在年龄、性别、肿块大小、肿块位置、分化程度、淋巴结转移以及TNM分期中差异均无统计学意义(P>0.05)。结论:miRNA-100在胃癌中的异常表达可能与胃癌的发生有关。
Objective： To detect the expression of microRNA-100（ miRNA-100） in gastric cancer and its relation with clinicopathologic features. Methods： The total RNA in 50 gastric cancer tissue specimens and non-tumor adjacent tissue specimens were extracted,the expression levels of miRNA-100 were detected by Taq Man real-time quantitative PCR assay. The correlations of miRNA-100 expression and differentiation and lymph node metastasis of gastric cancer were analyzed. Results： The expression levels of miRNA-100 in gastric carcinoma tissue and normal gastric tissue were 1. 31 ± 0. 69 and 0. 74 ± 0. 36,respectively,the difference of which was statistical significance（ P〈0. 01）. The differences of miRNA-100 expressions in age,gender,tumor location,tumor size,degree of differentiation,lymph node metastasis and TNM staging were not statistically significant（ P〉0. 05）. Conclusions： The abnormal expression of miRNA-100 may be associated with the occurrence of gastric cancer.
出处
《蚌埠医学院学报》
CAS
2015年第1期37-39,42,共4页
Journal of Bengbu Medical College
